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Behaviour of cold-adapted Listeria monocytogenes under conditions representative of meat processing plants Open Access


Other title
Listeria monocytogenes
flow cytometry
Type of item
Degree grantor
University of Alberta
Author or creator
Vail, Kathleen M
Supervisor and department
McMullen, Lynn (Agricultural, Food and Nutritional Science)
Examining committee member and department
Keelan, Monika (Lab Medicine & Pathology)
Jones, Tineke (Agriculture and Agri-Food Canada)
Gaenzle, Michael (Agricultural, Food and Nutritional Sciences)
Department of Agricultural, Food, and Nutritional Science
Food Science and Technology
Date accepted
Graduation date
Master of Science
Degree level
The objective of this study was to examine filamentation characteristics of cold-adapted Listeria monocytogenes under conditions representative of a meat processing plant. Initial analysis of 16 strains found three strains with a wide range of filamentation characteristics and these were used in subsequent experiments. L. monocytogenes were grown at 3°C in Tryptic Soy Broth (TSB), TSB with 4% or 8% NaCl, TSB adjusted to pH 5.5, 6 or 8.5, and TSB with 4% NaCl adjusted to pH 6. Filamentation characteristics were determined with microscopy and flow cytometry. Filamentation in saline conditions peaked early in the logarithmic phase whereas in acidic conditions filamentation increased steadily over time. In alkaline media filamentation peaked twice during the growth cycle. A combination of acidic and saline conditions resulted in an initial peak, followed by gradual increase of filamentation over time. The length of filaments increased with an increase in stressful conditions.
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