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Permanent link (DOI): https://doi.org/10.7939/R3M929

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Development of Rapid Techniques for Healthcare and Environment Monitoring Open Access

Descriptions

Other title
Subject/Keyword
Lab on a Chip
microchannel
mobile water kit
coliform
cantilever
microwells
troponin
Biofunctionalization
myoglobin
rapid
dielectrophoresis
heart attack
environment
enzymatic reaction
E.coli
Water
antibody
healthcare
micromixers
microfluidics
biosensor
micropillars
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Gunda, Naga Siva Kumar
Supervisor and department
Mitra, Sushanta (Mechanical Engineering)
Examining committee member and department
Bhiladvala, Rustom (Mechanical Engineering)
Kumar, Aloke (Mechanical Engineering)
Thundat, Thomas (Chemical and Materials Engineering)
Ghaemi, Sina (Mechanical Engineering)
Department
Department of Mechanical Engineering
Specialization

Date accepted
2014-04-02T13:09:26Z
Graduation date
2014-06
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
The development of rapid detection techniques to be applied at the point of use, has been gaining significant attention in the recent years. Rapid techniques are important and preferable methods in healthcare and environment sector due to the advantages they offer such as low-cost and reduced requirement of resources, both in terms of material and personnel. They are of utmost importance particularly in rural settings, where the facilities are inadequate. The main focus of the present thesis is to develop and assess the applicability, efficacy and advantages of such rapid techniques for healthcare and environment monitoring. Two problems have been considered to address the requirement for rapid techniques, one from healthcare monitoring sector (cardiac marker detection) and another one from environmental sector (water-borne pathogen detection). The first problem involves the development of rapid microfluidic based methods for the detection of cardiac markers (myoglobin and troponin), in order to predict the on-set of myocardial infarction. The second problem involves the development of a field test kit for the rapid detection of total coliform and Escherichia coli (E.coli) in potable water in order to address the needs of environmental monitoring. The rapid detection techniques presented in this thesis are simple, efficient, easy to use, inexpensive, reliable and have a great potential to be applied at the point of use.
Language
English
DOI
doi:10.7939/R3M929
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
N. S. K. Gunda, M. Singh, L. Norman, K. Kaur and S. K. Mitra, “Optimization and characterization of biomolecule immobilization on silicon substrates using (3-aminopropyl)triethoxy silane (APTES) and glutaraldehyde linker”, Applied Surface Science, In Press, 2013.N. S. K. Gunda and S. K. Mitra, “Challenges and Opportunities for Capillary Based Biofunctionalization of Microcantilever Arrays”, Journal of Electrochemical Society, 161 (2), B3167, 2014.N. S. K. Gunda, S. Bhattacharjee, and S. K. Mitra,"Study on the use of dielectrophoresis and electrothermal forces to produce on-chip micromixers and microconcentrators", Biomicrofluidics, 6, 034118, 2012.N. S. K. Gunda and S. K. Mitra, “Modeling of Dielectrophoretic Transport of Myoglobin Molecules in Microchannels”, Biomicrofluidics, 4, 014105, 2010.N. S. K. Gunda and S. K. Mitra, “Dielectrophoresis for manipulation of biomolecules”, Biomedical Engineering/Book 4, ISBN 978-953-307-557-0, 2011.N. S. K. Gunda, S. Naciker, M. S. Ghoraishi, S. Bhattacharjee, T. Thundat and S. K. Mitra, “Enhanced rapid detection of total coliform and E.coli in contaminated water samples using specifically formulated dual enzyme substrate chemical reagents”, Water Research, Under Review, 2014.N. S. K. Gunda, S. Naciker, S. Shinde, S. Kimbahune, S. Shrivastava, and S. K. Mitra, “Mobile Water Kit (MWK): A Low-Cost Water Monitoring System Integrated with Smartphone for Rapid Detection of Total Coliform and E.coli”, Analyst, Under Review, 2014.N. S. K. Gunda, S. Naicker, M. S. Ghoraishi, S. Bhattacharjee, T. G. Thundat, and S. K. Mitra, “Rapid Detection of Total Coliform and E.Coli in Contaminated Water Using Chemically Modified Microwells”, Paper # J4-2788, Proceedings of 224th ECS Meeting, San Francisco, USA, October 27 to November 1, 2013.N. S. K. Gunda and S. K. Mitra, “Distinguished Invited Speaker--Capillary-Based Assay for Cardiac Markers With Cantilever Platform”, Paper # J4-2792, Proceedings of 224th ECS Meeting, San Francisco, USA, October 27 to November 1, 2013.N. S. K. Gunda and S. K. Mitra, “Microfluidic Based Biosensor for Detection of Cardiac Markers”, Paper # FEDSM2013-16270, Proceedings of ASME 2013 Fluids Engineering Summer Meeting, Incline Village, Nevada, USA, July 7-11, 2013.N. S. K. Gunda, S. Naicker, M. S. Ghoraishi, S. Bhattacharjee, T. G. Thundat and S. K. Mitra, “Microspot with Integrated Wells (MSIW) for the detection of E.coli”, Paper # ICNMM2013-73037, Proceedings of ASME 2013 11th International Conference on Nanochannels, Microchannels, and Minichannels, Sapporo, Japan, June 16-19, 2013.N. S. K. Gunda, S. Naicker, and S. K. Mitra, “Electrochemical detection of cardiac myoglobin using microchannel with interdigitated electrodes (MCIE)”, Paper # J2-1473, Proceedings of 223rd ECS Meeting, Toronto, Canada, May 12-17, 2013.N. S. K. Gunda, M. Singh, S. S. Roy, and S. K. Mitra, “Developing Electrochemical Impedance Immunosensor for the Detection of Myoglobin in Blood Serum”, IMCS 2012 - The 14th International Meeting on Chemical Sensors, Nürnberg/Nuremberg, Germany, May 20 - 23, 2012.N. S. K. Gunda and S. K. Mitra, “Microfluidics for Detection of Myoglobin in Blood Sample”, Paper # E2-1167R, Proceedings of 219th ECS Meeting, Montreal, Canada, May 1-6, 2011.N. S. K. Gunda and S. K. Mitra, “Experimental Investigation of Dielectrophoretic Behavior of Myoglobin and Silica Particles on a Microelectrode Chip”, Paper # FEDSM-ICNMM2010-30306, Proceedings of ASME 2010 3rd Joint US-European Fluids Engineering Summer Meeting and 8th International Conference on Nanochannels, Microchannels, and Minichannels, FEDSM2010-ICNMM2010, Montreal, Canada, August 2-4, 2010.N. S. K. Gunda and S. K. Mitra, “Modeling of Dielectrophoresis for Myoglobin Molecules in a Microchannel with Parallel Electrodes”, Paper # IMECE2009-10765, Proceedings of the ASME 2009 International Mechanical Engineering Congress and Exposition, IMECE09, Lake Buena Vista, Florida, USA, November 13-19, 2009.N. S. K. Gunda, S. K. Mitra and S. Bhattacharjee, “Dielectrophoretic Mixing with Novel Electrode Geometry”, Paper # FEDSM2009-78260, Proceedings of the ASME 2009 Fluids Engineering Division Summer Meeting, FEDSM09, Vail, Colorado, August 2-6, 2009.S. K. Mitra, N. S. K. Gunda, S. Naciker, M. S. Ghoraishi, S. Bhattacharjee, T. Thundat, “Enhanced rapid detection of E.coli”. ROI No. 2013040 TEC Edmonton, Alberta, Canada, 2013.S. K. Mitra, N. S. K. Gunda, S. Naicker, M. Ghoraishi, S. Bhattacharjee, and T. Thundat, “Microspot with integrated wells for detection of E. coli”, ROI No. 2013041, 2013.S. K. Mitra, N. S. K. Gunda, and S. Naicker, “Mobile Water Kit (MWK) for Rapid Detection of Total Coliform and E. coli”, ROI No. 2013084, 2014.

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