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Permanent link (DOI): https://doi.org/10.7939/R33J39874

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Study of Non-Covalent Multisubunit Protein-Carbohydrate Interactions by Electrospray Ionization Mass Spectrometry Open Access

Descriptions

Other title
Subject/Keyword
Protein-Carbohydrate Interactions
ESI-MS
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Zhang,Yixuan
Supervisor and department
Klassen, John (Chemistry)
Examining committee member and department
Cairo, Christopher (Chemistry)
McDermott, Mark (Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2013-07-05T09:22:37Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
This thesis describes the development and application of electrospray ionization mass spectrometry (ESI-MS) based techniques to investigate protein-carbohydrate interactions in vitro. A catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) assay was developed for the identification of specific interactions between water-soluble multisubunit proteins and glycosphingolipids (GSL). The assay is of high sensitivity and specificity, and demonstrates the potential for discovering biologically relevant protein-GSL interactions. Collision-induced dissociation (CID) experiments and molecular dynamic simulations were performed to investigate the dissociation pathways of multisubunit protein-ligand complexes in the gas phase. The observation of multiple dissociation pathways suggests that collisional activation of multisubunit protein-ligand complexes in the gas phase is likely to induce significant changes to the nature of the protein-ligand interactions.
Language
English
DOI
doi:10.7939/R33J39874
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Zhang, Y.; Liu, L.; Daneshfar, R.; Kitova, E. N.; Li, C.; Jia, F.; Cairo, C. W.; Klassen, J. S. Anal. Chem. 2012, 84(18), 7618-21.Zhang, Y.; Deng, L.; Kitova, E.N.; Klassen, J.S. J. Am. Soc. Mass. Spectrom. Accepted.

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