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Permanent link (DOI): https://doi.org/10.7939/R3D50G33W

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Modeling of Integrated Microfluidics-CMOS Lab-on-Chip Technology Open Access

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Other title
Subject/Keyword
lab on a chip
modeling
lab on chip
polymer
magnetic
point of care
microfluidics
thermal
LOC
diagnostic
CMOS
polymerase chain reaction
sample preparation
microfluidic
finite element
bead
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Caverhill-Godkewitsch, Saul A.
Supervisor and department
Sameoto, Dan (Mechanical Engineering, University of Alberta)
Backhouse, Chris (Electrical and Computer Engineering, University of Waterloo)
Elliott, Duncan (Electrical and Computer Engineering, University of Alberta)
Examining committee member and department
Backhouse, Chris (Electrical and Computer Engineering, University of Waterloo)
Elliott, Duncan (Electrical and Computer Engineering, University of Alberta)
Dinavahi, Venkata (Electrical and Computer Engineering, University of Alberta)
Dubljevic, Stevan (Chemical and Materials Engineering, University of Alberta)
Sameoto, Dan (Mechanical Engineering, University of Alberta)
Department
Department of Electrical and Computer Engineering
Specialization
Biomedical Engineering
Date accepted
2015-09-25T14:58:55Z
Graduation date
2015-11
Degree
Master of Science
Degree level
Master's
Abstract
The infrastructure necessary to support diagnostic and pathogen-detection processes does not exist in some regions of the world that need it most. Access to fast, inexpensive and portable diagnostic infrastructure could be a solution to this problem. Next generation lab-on-chip (LOC) systems that integrate polymer microfluidics with CMOS circuit instrumentation can replace the need for costly and immobile conventional diagnostic laboratories. Portable DNA-based tests require three components: sample preparation (SP), amplification and detection. Finite element analysis allows for complicated engineering designs to be modeled to determine real-world behaviour, allowing key parameters to be explored prior to fabrication, saving time and money during prototyping. The BioMEMS project group at the University of Alberta has been working on a series of integrated polymer microfluidic-CMOS platforms or "chips" as such LOC systems. Amplification is performed via the polymerase chain reaction (PCR) in our LOC platform. Significant updates to the SP and PCR modules of the three-step diagnostic process were required for the latest revision of the chip. By leveraging the power of Finite Element Analysis (FEA) modeling, an SP module capable of processing a 1 μL sample in minutes was designed and built. Similarly, PCR heaters were designed that generate ±0.5 ◦C uniformity at a target temperature of 95 ◦C in the reaction chamber volume while enabling a 3-step PCR protocol of 35 cycles (4.19 seconds per cycle) in under two and a half minutes. These achievements are a step towards altering the current state of inaccessible point of care (POC) diagnostics.
Language
English
DOI
doi:10.7939/R3D50G33W
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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