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Permanent link (DOI): https://doi.org/10.7939/R3833N67F

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Isotope Labeling Liquid Chromatography Mass Spectrometry: Metabolite Identification and Human Salivary Metabolome Profiling Open Access

Descriptions

Other title
Subject/Keyword
Mass Spectrometry
Metabolomics
Saliva
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Zheng, Jiamin
Supervisor and department
Li, Liang (Chemistry)
Examining committee member and department
Dixon, Roger (Psychology)
Harrison, Jed (Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2012-09-26T13:31:53Z
Graduation date
2012-09
Degree
Master of Science
Degree level
Master's
Abstract
The objective of this work was to use paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust way of mass spectrometry (MS)-based metabolome profiling. A differential 13C-/12C-isotope dansyl chloride (DnsCl) derivatization strategy has been further developed and applied for qualitative and quantitative profiling of amine- and phenol-containing metabolites by liquid chromatography electrospray ionization Fourier Transform ion cyclotron resonance MS (LC-ESI FT-ICR-MS). For labeled metabolites, a new technique using skimmer-region fragmentation, followed by tandem MS analysis of formed fragment ions, was developed for generating structural information. In addition, a new method based on use of this isotope labeling LC-MS platform was developed for human salivary metabolome analysis. It offered superior performance over other reported methods for saliva metabolome profiling. Its potential utility for disease biomarker discovery was demonstrated in the analysis of metabolomic differences between normal individuals and diseased-individuals with mild cognitive impairment (MCI).
Language
English
DOI
doi:10.7939/R3833N67F
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Jiamin Zheng and Liang Li, “Fragmentation of Protonated Dansyl-labeled Amines for Structural Analysis of Amine-containing Metabolites”, Int. J. Mass Spectrom, 2012, 316-318, 292-299.

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