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Permanent link (DOI): https://doi.org/10.7939/R3JD80

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Biochemical identification of bacteriocins from Enterococcus faecalis 710C Open Access

Descriptions

Other title
Subject/Keyword
Mass spectrometry
Lactic acid bacteria
Bacteriocin
Food safety
Antimicrobial peptide
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Liu, Xiaoji
Supervisor and department
McMullen, Lynn (Agricultural, Food and Nutritional Science)
Examining committee member and department
Michael, Gänzle (Agricultural, Food and Nutritional Science)
McMullen, Lynn (Agricultural, Food and Nutritional Science)
Vederas, John (Chemistry)
Department
Department of Agricultural, Food and Nutritional Science
Specialization

Date accepted
2010-01-07T17:50:06Z
Graduation date
2010-06
Degree
Master of Science
Degree level
Master's
Abstract
Enterococcus faecalis 710C is a lactic acid bacterium that produces two bacterocins, ent7A and ent7B. Both ent7A and ent7B have strong activity against gram-positive food pathogens including Listeria spp., Clostridium spp., vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus aureus (MRSA). Mass spectrometry analyses revealed that both ent7A (5201 Da) and ent7B (5207 Da) have formylated N-terminal methionine. The amino acid sequences, structural gene sequences of ent7 from nucleotide position 1-275 and immunity gene were determined. Circular dichroism data suggest that in aqueous solution ent7A and ent7B have 20 to 25% alpha-helical region. Addition of membrane-mimicking reagent (trifluoroethanol) did not significantly enhance the alpha-helical content in ent7A and ent7B. Chiral analysis by gas chromatography- mass spectrometry showed that the amino acid residues elucidated in ent7A and ent7B were all in L-configuration.
Language
English
DOI
doi:10.7939/R3JD80
Rights
License granted by Xiaoji Liu (xiaoji2@ualberta.ca) on 2010-01-07T17:36:55Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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