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Permanent link (DOI): https://doi.org/10.7939/R35640

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Exploring the role of sialic acid in the glycoprotein LFA-1 using bioconjugate chemistry Open Access

Descriptions

Other title
Subject/Keyword
LFA-1
integrin glycosylation
biconjugate chemistry
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Sadek, Christopher
Supervisor and department
Cairo, Christopher (Chemistry)
Examining committee member and department
Lowary, Todd (Chemistry)
Kane, Kevin (Medical Microbiology)
Cairo, Christopher (Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2012-05-31T09:33:21Z
Graduation date
2011-06
Degree
Master of Science
Degree level
Master's
Abstract
The lymphocyte function associated antigen-I (LFA-1) is important to a variety of immune cell processes including immune synapse formation and lymphocyte homing. Studies have shown that lymphocytes exhibit increased adhesion upon sialic acid removal by neuraminidase. Recent studies of β1 integrin function have shown a role for sialic acid. We hypothesized that modifications to sialic acids on LFA-1 may influence ligand binding, as a potential mechanism responsible for the neuraminidase effect. Herein, we describe progress towards studying LFA-1 sialic acids and their role in LFA-1 mediated adhesion. We describe applications of metabolic labeling strategies. We demonstrate bioconjugation of alkynyl drag-tags for resolution of sialoforms of metabolically-labeled murine IgG. We demonstrate bioconjugation of synthetic carbohydrate epitopes to an azide-containing model protein by Staudinger ligation. Finally, we report the production of stable HeLa cell lines expressing GFP-fused NEU3, as a model system for in vivo studies of NEU3.
Language
English
DOI
doi:10.7939/R35640
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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