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Permanent link (DOI): https://doi.org/10.7939/R3037J

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Rab32 family proteins and their interactors Open Access

Descriptions

Other title
Subject/Keyword
Rab29
Rab32
evolution
MAM
Rab38
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Ortiz Sandoval, Carolina Guadalupe
Supervisor and department
Simmen, Thomas
Examining committee member and department
Dacks, Joel (Cell Biology)
Melançon, Paul (Cell Biology)
Nargang, Frank (Biological Sciences)
Department
Department of Cell Biology
Specialization

Date accepted
2013-09-26T14:52:56Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
The ER mitochondria-associated membrane (MAM) is a hub for many calcium-mediated processes required for cell survival, including apoptosis, autophagy, and ATP production. In this thesis, we identified and characterized the interaction between MAM-enriched Rab32 and its effector Drp1, a master regulator of mitochondrial fission. Evolutionary studies of Rab32 suggest that it forms a family with Rab38 and Rab29. While only Rab32 and Rab38 participate in melanosome trafficking, all family members interact with Drp1 to influence the mitochondria phenotype, Rab32 being the stronger interactor, followed by Rab38 and Rab29. Moreover, I was also able to determine that Rab32 interacts with syntaxin-17, a MAM-enriched SNARE protein which, like Rab32, participates in autophagy; Rab29 showed a weaker interaction with this SNARE, while this interaction was not seen with Rab38. Lastly, phylogenetic studies indicate that Rab29 and Rab38 are both a result of Holozoan expansion of Rab32, and that Rab29 diverged earlier in evolution from Rab32 than Rab38.
Language
English
DOI
doi:10.7939/R3037J
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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