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Permanent link (DOI): https://doi.org/10.7939/R39K45W8M

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Epitope Specificities of the Groups Y and W-135 Polysaccharides of Neisseria meningitides. Open Access

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Author or creator
Moore, S. L.
Uitz, C.
Ling, C.-C.
Bundle, D. R.
Fusco P. C.
Michon, F.
Additional contributors
Subject/Keyword
epitopes
bacterial immunology
polysaccharides
antigens
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Previous studies have identified the length dependency of several polysaccharide (PS) protective epitopes. We have investigated whether meningococcal polysaccharides Y and W-135 possess such epitopes. Oligosaccharides (OSs) consisting of one or more disaccharide repeating units (RU) were derived from the capsular PSs of group Y and W-135 meningococci (GYMP and GWMP, respectively) by mild acid hydrolysis. The relative affinities of anticapsular antibodies binding to derivative OSs of different chain lengths were measured in inhibition enzyme-linked immunosorbent assays. As OS size increased from two to three RU, there was a notable increase in binding inhibition of rabbit anti-group Y antiserum. This pattern of antibody binding inhibition was also observed for rabbit antiserum to group W-135, though the inhibition increase was much more pronounced. In the cases of both OS species, the concentration of inhibiting antigen required to achieve 50% inhibition of rabbit immunoglobulin binding increased progressively as the inhibiting disaccharide chain length increased from 1 RU through greater than 50 RU. These data suggest that antibodies directed against both of these meningococcal PSs recognize conformational epitopes only fully expressed in higher-molecularweight forms of these antigens.
Date created
2007
DOI
doi:10.7939/R39K45W8M
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© 2007 American Society for Microbiology. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
Citation for previous publication
Moore, S. L., Uitz, C., Ling, C.-C., Bundle, D. R., Fusco P. C. and Michon, F. Epitope Specificities of the Groups Y and W-135 Polysaccharides of Neisseria meningitides. Clinical and Vaccine Immunology, 14, 1311-1317, 2007.
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