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Permanent link (DOI): https://doi.org/10.7939/R3G84G

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Accumulation of Al in Root Mucilage of an Al-Resistant and an Al-Sensitive Cultivar of Wheat Open Access

Descriptions

Author or creator
Archambault, D. J.
Zhang, G.
Taylor, G. J.
Additional contributors
Subject/Keyword
Exudation
Excised Roots
Acid
Localization
Mechanism
Apices
Cell-division
Triticum-Aestivum L
Malate
Aluminum-tolerance
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
To estimate rates of Al accumulation within the symplasm, all apoplastic pools of Al need to be eliminated or accounted for. We have developed a revised kinetic protocol that allows us to estimate the contribution of mucilage-bound Al to total, nonexchangeable Al, and to eliminate the mucilage as an apoplastic pool of Al. By comparing the Al content of excised root tips (2 cm) of wheat ( Triticum aestivum L.) with and without the removal of the mucilage (using a 10-min wash in 1 M NH4Cl), we found that Al bound to the mucilage accounted for approximately 25 to 35% of Al remaining after desorption in citric acid. The kinetics of Al uptake into mucilage were biphasic, with a rapid phase occurring in the first 30 min of uptake, followed by a linear phase occurring in the remainder of the experimental period (180 min). By adopting a step for removal of mucilage into our existing kinetic protocol, we have been able to isolate a linear phase of uptake with only a slight deviation from linearity in the first 5 min. Although we cannot unambiguously identify this phase of uptake as uptake into the symplasm, we believe this new protocol provides us with the most accurate quantitative estimate of symplastic Al yet available.
Date created
1996
DOI
doi:10.7939/R3G84G
License information
Rights
© 1996 American Society of Plant Biologists. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
Citation for previous publication
Archambault, D.J., Zhang, G. & Taylor, G.J. (1996). Accumulation of Al in Root Mucilage of an Al-Resistant and an Al-Sensitive Cultivar of Wheat. Plant Physiology, 112(4), 1471-1478.
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