ERA

Download the full-sized PDF of High-throughput, high-sensitivity analysis of gene expression in ArabidopsisDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R38K75327

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Biological Sciences, Department of

Collections

This file is in the following collections:

Journal Articles (Biological Sciences)

High-throughput, high-sensitivity analysis of gene expression in Arabidopsis Open Access

Descriptions

Author or creator
Kris, R. M.
Felder, S.
Deyholos, M.
Lambert, G. M.
Hinton, J.
Botros, I.
Martel, R.
Seligmann, B.
Galbraith, D. W.
Additional contributors
Subject/Keyword
Genome
Oxidative Stress
Length CDNA Microarray
High-Salinity
Array
Pathways
Responsive Genes
Discovery
Profiles
Thaliana
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
High-throughput gene expression analysis of genes expressed during salt stress was performed using a novel multiplexed quantitative nuclease protection assay that involves customized DNA microarrays printed within the individual wells of 96-well plates. The levels of expression of the transcripts from 16 different genes were quantified within crude homogenates prepared from Arabidopsis (Arabidopsis thaliana) plants also grown in a 96-well plate format. Examples are provided of the high degree of reproducibility of quantitative dose-response data and of the sensitivity of detection of changes in gene expression within limiting amounts of tissue. The lack of requirement for RNA purification renders the assay particularly suited for high-throughput gene expression analysis and for the discovery of novel chemical compounds that specifically modulate the expression of endogenous target genes.
Date created
2007
DOI
doi:10.7939/R38K75327
License information
Rights
© 2007 American Society of Plant Biologists. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
Citation for previous publication
Kris, R. M., Felder, S., Deyholos, M., Lambert, G. M., Hinton, J., Botros, I., Martel, R., Seligmann, B., & Galbraith, D. W. (2007). High-throughput, high-sensitivity analysis of gene expression in Arabidopsis. Plant Physiology, 144(3), 1256-1266. doi: 10.1104/pp.107.098681.
Source
Link to related item

File Details

Date Uploaded
Date Modified
2014-04-28T21:30:39.653+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1105660
Last modified: 2015:10:12 20:49:49-06:00
Filename: PP_144_2007_1256.pdf
Original checksum: a11547162ee6b9f8770a2df51286bd6f
Well formed: true
Valid: true
File title: pp098681 1256..1266
Page count: 11
Activity of users you follow
User Activity Date