Aquaporin regulation in poplar and spruce trees under environmental change. Open Access
- Other title
radial water flow
leaf hydraulic conductance
- Type of item
- Degree grantor
University of Alberta
- Author or creator
- Supervisor and department
Hacke, Uwe G (Department of Renewable Resources)
- Examining committee member and department
Maurel, Christophe (UMR Biochimie et Physiologie Moleculaire des plantes - INRA SupAgro - Montpellier - FRANCE)
Comeau, Phil (Department of Renewable Resources)
Scarpella, Enrico (Department of Biological Sciences)
Zwiazek, Janusz (Department of Renewable Resources)
Department of Renewable Resources
Forest Biology and Management
- Date accepted
- Graduation date
Doctor of Philosophy
- Degree level
This dissertation describes a series of experiments that examined: 1) hydraulic responses of Populus trichocarpa x deltoides, Populus trichocarpa and Picea glauca plants to change in their surrounding environment; 2) Changes of aquaporin expression in response to such changes.
In the first study, we demonstrated that changes of the transpirational demand is related to fine adjustment of root water uptake that is associated with up-regulation of plasma membrane intrinsic proteins isoforms (PIPs) in hybrid poplar saplings. PIP1 proteins are mostly localized in the endodermis where they may facilitate water movement to the stele. In the second study, we investigated the dynamics of leaf hydraulics in P. trichocarpa saplings exposed to a dehydration-rewatering episode. Fast leaf recovery was associated with an increase in expression of several tonoplast intrinsic proteins isoforms (TIPs) localized in xylem parenchyma. In the third study, we considered the physiological importance of foliar water uptake in P. glauca plants exposed to drought. In order to study the role of aquaporin in needle water uptake, we characterized the aquaporin family in white spruce. Our findings are consistent with the hypothesis that aquaporins facilitate radial water movement from the atmosphere towards the needle vascular tissue, therefore providing an alternate water source for embolism repair in conifers.
These results suggest the several roles of aquaporin regulation in the dynamic and fine adjustment of tree-water relations.
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- Citation for previous publication
J. Laur and U. G. Hacke, “Transpirational demand affects aquaporin expression in poplar roots.” Journal of Experimental Botany, vol. 64, 2283-2293.J. Laur and U. G. Hacke, “Exploring Picea glauca aquaporins in the context of needle water uptake and xylem refilling.” New Phytologist, vol. 203, 388-400
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