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Permanent link (DOI): https://doi.org/10.7939/R3HH8Q

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Using designed zinc finger proteins to inhibit hepatitis B virus transcription in tissue culture Open Access

Descriptions

Other title
Subject/Keyword
hepatitis B virus
tissue culture
inhibit transcription
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Miller, Kristen L
Supervisor and department
Tyrrell, D. Lorne (Medical Microbiology and Immunology)
Examining committee member and department
Schang, Luis (Biochemistry)
Houghton, Michael (Medical Microbiology and Immunology)
Tyrrell, D. Lorne (Medical Microbiology and Immunology)
Evans, David (Medical Microbiology and Immunology)
Department
Department of Medical Microbiology and Immunology
Specialization
Virology
Date accepted
2012-08-24T10:06:44Z
Graduation date
2012-11
Degree
Master of Science
Degree level
Master's
Abstract
Upon infection with hepatitis B virus (HBV) the result will be either a chronic infection or clearance of the virus. For the chronic carriers of HBV, therapy is primarily limited to nucleoside analogs, which inhibits viral replication and prevents production of progeny virus, however the nucleoside analogs have little affect on the reservoir of the virus in the nucleus, the covalently closed circular DNA (cccDNA). Our lab previously designed zinc finger proteins (ZFPs) to target sequences within the duck hepatitis B virus (DHBV) and HBV cccDNAs to inihibit hepadnaviral replication. This is a novel approach to treatment of HBV infection because the reservoir of the virus is directly targeted. The DHBV-specific ZFPs have been shown to inhibit DHBV replication in tissue culture. I have assessed the ability of HBV-specific ZFPs, delivered by lentiviral vectors, to inhibit HBV transcription in two HBV-infected human hepatoma cell lines. The currently designed ZFPs failed to suppress HBV replication.
Language
English
DOI
doi:10.7939/R3HH8Q
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File format: pdf (Portable Document Format)
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File size: 17575782
Last modified: 2015:10:12 17:26:38-06:00
Filename: Miller_Kristen_Fall 2012.pdf
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File title: FINAL Title page, abstract, abrev TOC.pdf
File title: Microsoft Word - FINAL Title page, abstract, abrev TOC.docx
File author: Kristen Miller
Page count: 189
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