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Permanent link (DOI): https://doi.org/10.7939/R3CP7T

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Investigating the Caspase Cleavage of the JunB Transcription Factor Open Access

Descriptions

Other title
Subject/Keyword
JunB
Apoptosis
AP-1
ALK
Lymphoma
Caspase
ALK+ ALCL
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Lee, Jason KH
Supervisor and department
Ingham, Robert (Medical Microbiology and Immunology)
Examining committee member and department
Ostergaard, Hanne (Medical Microbiology and Immunology)
Goping, Ing Swie (Biochemistry)
Smiley, James (Medical Microbiology and Immunology)
Barry, Michele (Medical Microbiology and Immunology)
Department
Department of Medical Microbiology and Immunology
Specialization
Immunology
Date accepted
2013-03-27T11:56:18Z
Graduation date
2013-06
Degree
Master of Science
Degree level
Master's
Abstract
The activation of caspases is an important step not only in apoptosis induction, but in cellular processes such as proliferation, differentiation, and the immune response. Here, we show that the AP-1 family transcription factor JunB is cleaved and dephosphorylated in a caspase-dependent manner in apoptotic cells. We demonstrate that JunB is cleaved directly by caspases, and identify aspartate 137 as the cleavage site. JunB cleavage separates the amino-terminal transactivation and carboxy-terminal DNA binding/dimerization domains to disrupt JunB transcriptional activity. Moreover, the carboxy-terminal cleavage fragment retains DNA binding activity, and the ability to dimerize with AP-1 proteins. This fragment interferes with full-length JunB binding to AP-1 sites and inhibits AP-1–dependent transcription. Finally, we show that the carboxy-terminal cleavage fragment impairs proliferation and promotes apoptosis when overexpressed in a T-cell lymphoma cell line. In summary, our findings reveal a novel mechanism of regulating the activity of an AP-1 family transcription factor.
Language
English
DOI
doi:10.7939/R3CP7T
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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