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Permanent link (DOI): https://doi.org/10.7939/R3HM52W41

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Whole-animal to molecular studies of solute transport in fishes Open Access

Descriptions

Other title
Subject/Keyword
sodium
homeostasis
physiology
dogfish
phosphate
hagfish
salinity
NHE
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Guffey, Samuel C
Supervisor and department
Goss, Greg (Biological Sciences)
Examining committee member and department
Chang, John (Biological Sciences)
Gallin, Warren (Biological Sciences)
Leys, Sally (Biological Sciences)
Department
Department of Biological Sciences
Specialization
Physiology, Cell, and Developmental Biology
Date accepted
2013-09-24T16:01:53Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
I have examined solute transport in two fishes at the whole-animal, tissue, and molecular levels. In the Pacific hagfish, I measured the kinetics of phosphate absorption in isolated gut, skin, and gill preparations, and implicated an NaPi-II transporter based on RT-PCR results and the kinetics of phosphate uptake. In a second series of experiments, subjecting Pacific dogfish to reduced salinity revealed that plasma sodium and chloride homeostasis are maintained. Therefore these ions are not a limiting factor in reduced salinity; however, urea efflux significantly increased over the 48 hour exposure. Finally, I investigated the molecular physiology of sodium homeostasis by cloning Na+/H+ Exchanger [NHE] isoforms 2 and 3 from the dogfish gill and expressing them in NHE-deficient AP-1 cell lines. The sensitivity to inhibitors was found to be different from mammalian NHEs, revealing appropriate concentrations to use for elasmobranchs and other fishes, and allowing future mechanistic studies to be conducted.
Language
English
DOI
doi:10.7939/R3HM52W41
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Guffey, S.C., and Goss, G.G. Comparative Biochemistry and Physiology A: Molecular and Integrative Physiology. (CBP A/2013/ms.22401).

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File author: Samuel C Guffey
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