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Mechanisms of cabbage seedpod weevil, Ceutorhynchus obstrictus, resistance associated with novel germplasm derived from Sinapis alba x Brassica napus Open Access

Descriptions

Other title
Subject/Keyword
insect-plant interactions
2-phenylethyl glucosinolate
host olfactory cues
weevil vision
associational resistance
1-methoxy-3-indolylmethyl glucosinolate
host visual cues
antixenosis
plant defence
cabbage seedpod weevil
antibiosis
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Tansey, James
Supervisor and department
Keddie, Andrew (Biological Sciences)
Dosdall, Lloyd (Agricultural, Food and Nutritional Science)
Examining committee member and department
Rahman, Habibur (Agricultural, Food and Nutritional Science)
Evenden, Maya (Biological Sciences)
Lamb, Robert J. (Research Scientist, Cereal Research Centre, Agriculture and Agri-Food Canada. Winnipeg, Manitoba; Adjunct Professor, University of Manitoba, Entomology)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization

Date accepted
2009-10-01T19:39:20Z
Graduation date
2009-11
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
The cabbage seedpod weevil, Ceutorhynchus obstrictus (Marsham) (Coleoptera: Curculionidae), is an important pest of brassicaceous oilseed crops, especially canola (Brassica napus L. and Brassica rapa L.) in North America and Europe. Application of foliar insecticide is the only method currently employed to control C. obstrictus populations; because this approach is environmentally unsustainable, alternatives including host plant resistance have been explored. White mustard, Sinapis alba L., is resistant to C. obstrictus and was chosen as a potential source of resistance for B. napus oilseed. Interspecific crosses of S. alba x B. napus have produced several lines that are resistant to C. obstrictus feeding and oviposition and yield fewer, lighter-weight weevil larvae that take longer to develop. I investigated potential mechanisms of this resistance, including assessing differences in visual and olfactory cues among resistant and susceptible genotypes, and antixenosis and antibiosis. Determining effects of visual cues associated with host plant resistance required investigation of weevil vision. Deployment strategies for resistant germplasm were assessed to evaluate incorporation of susceptible refugia to promote long-term durability of resistance traits. Results reported in Chapter 2 indicate that the C. obstrictus visual system is apparently trichromatic and incorporates receptors with response maxima near 350, 450, and 550 nm. Modelling indicated that UV light alone reduced weevil responses but the interaction of yellow and UV light increased responses at a threshold reflectance level of UV. Results reported in Chapter 3 indicated that differences in yellow and UV reflectance among host plant flowers influence host selection in C. obstrictus. Results described in Chapter 4 determine differential attraction to the odours of S. alba and B. napus and among resistant and susceptible accessions. Inferences of the identities of glucosinolates found in varying amounts among susceptible and resistant genotypes suggested that 2-phenylethyl glucosinolate influenced attractiveness. Results described in Chapter 5 indicate differences in adult feeding and oviposition preferences among resistant and susceptible genotypes. Oocyte development, larval biomass and larval development time varied among weevils feeding on resistant and susceptible genotypes. Based on results of Chapter 4, 1-methoxy-3-indolylmethyl glucosinolate was implicated as contributing to antixenosis and antibiosis resistance. Results reported in Chapter 6 describe effects of mixed plots of resistant and susceptible genotypes on weevil spatial distribution and oviposition. These results are consistent with associational resistance and attributed to reduced apparency of susceptible plants in mixtures and antixenosis resistance associated with resistant germplasm.
Language
English
DOI
doi:10.7939/R3MP9R
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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