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Permanent link (DOI): https://doi.org/10.7939/R30W7G

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Insights into the nuclear localization of Scalloped Open Access

Descriptions

Other title
Subject/Keyword
Nuclear localization
TEA domain
Scalloped
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Magico, Adam
Supervisor and department
Examining committee member and department
Department
Biological Sciences
Specialization

Date accepted
2011-08-25T19:07:32Z
Graduation date
2011-11
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
The Drosophila melanogaster protein Scalloped (Sd) is a member of the TEA/ATTS family of transcription factors, present throughout Eukarya. The protein is best known for its involvement in the development of the wing imaginal disc of Drosophila, although roles in nervous system, muscle (both cardiac and somatic), eye and leg development as well as the control of cellular proliferation have also been shown or predicted. Scalloped itself lacks a transcriptional activation domain and thus is thought to rely on binding with transcription intermediary factors (TIFs) which have one or more of these domains. Under this paradigm it is thought that Sd facilitates nuclear localization and targeted DNA binding of the Sd/TIF complex, while the TIF activates the target gene(s). In order to understand the mechanisms behind the nuclear translocation of Sd, it is demonstrated that a candidate bipartite nuclear localization signal is functional in S2 cells, and further that the region containing this signal is critical for Sd function during wing development. Evidence for the presence of a nuclear export signal is also given. Finally, a broad region of the C-terminal domain of Sd is identified as also being required for the proper nuclear localization of the protein. Although several TIFs of Sd have been identified, there are several lines of evidence which make it likely there are others yet to be discovered. Identifying these new factors would shed light on the function of Sd within whichever tissue a new TIF is discovered. With this in mind a candidate-gene approach was used to identify Drosophila vestigial-like 4 (dvgl-4). Herein it is demonstrated that dVgl-4 contains two putative Sd interacting domains and is able to interact with Sd in vitro and ex vivo. It is also shown that this protein is able to act in a dominant negative fashion during wing development, and that there are likely two isoforms of mRNA expressed from this gene, and that the expression of each is likely under the control of different promoters. Finally, over-expression phenotypes are described for several tissues in order to begin elucidating a potential function for dVgl-4 in Drosophila development.
Language
English
DOI
doi:10.7939/R30W7G
Rights
License granted by Adam Magico (puchailo@ualberta.ca) on 2011-08-23T15:24:51Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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