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Redox-regulated RNA helicase expression Open Access

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Author or creator
Kujat, S. L.
Owttrim, G. W.
Additional contributors
Subject/Keyword
Transcription
Photosynthesis
Mustard Sinapsis-Alba
Plastoquinone Pool
Protein-Phosphorylation
Cyanobacterium Synechocystis-6803
Light
Gene-Expression
PSBA Messenger-RNA
Chloroplast
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
In photosynthetic organisms it is becoming increasingly evident that light-driven shifts in redox potential act as a sensor that initiates alterations in gene expression at both the level of transcription and translation. This report provides evidence that the expression of a cyanobacterial RNA helicase gene, crhR, is controlled at the level of transcription and mRNA stability by a complex series of interacting mechanisms that are redox regulated. Transcript accumulation correlates with reduction of the electron transport chain between QA in photosystem II and QO in cyt b6f, when Synechocystis sp. strain PCC 6803 is cultured photoautotrophically or photomixotrophically and subjected to darkness and/or electron transport inhibitors or illumination that preferentially excites photosystem II. crhR mRNA stability is also regulated by a redox responsive mechanism, which differs from that affecting accumulation and does not involve signaling initiated by photoreceptors. The data are most consistent with plastoquinol/cyt b6 f interaction as the sensor initiating a signal transduction cascade resulting in accumulation of the crhR transcript. Functionally, CrhR RNA unwinding could act as a linker between redox regulated transcription and translation. The potential for translational regulation of redox-induced gene expression through RNA helicase-catalyzed modulation of RNA secondary structure is discussed.
Date created
2000
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© 2000 American Society of Plant Biologists. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
Citation for previous publication
Kujat, S.L. & Owttrim, G.W. (2000). Redox-regulated RNA helicase expression. Plant Physiology, 124(2), 703-713. doi: 10.1104/pp.124.2.703.
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