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Permanent link (DOI): https://doi.org/10.7939/R32G8B

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Broccoli sprout supplementation during placental insufficiency confers structural and functional neuroprotection to the fetal rat Open Access

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Other title
Subject/Keyword
brain
neonatal
broccoli
behavior
hypoxia-ischemia
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Black, Amy Maxine
Supervisor and department
Yager, Jerome (Pediatric Neurology)
Todd, Kathryn (Psychiatry)
Examining committee member and department
Colbourne, Fred (Psychology)
Department
Centre for Neuroscience
Specialization

Date accepted
2010-01-08T16:24:16Z
Graduation date
2010-06
Degree
Master of Science
Degree level
Master's
Abstract
Background: Perinatal ischemic brain injury leads to developmental disability (DD), which accounts for 30% of disabilities in children. Antepartum risk, or risk occurring prior to birth occurs in more than 90% of cases. This study investigated whether maternal ingestion of a natural health product (broccoli sprouts) would provide neuroprotection in an intrauterine model of HI. Methods: Intrauterine ischemia was induced by bilateral uterine artery ligation (BUAL) on E20 of gestation. Rats were fed broccoli sprouts (200 mg) from E15 until postnatal day 14 (PD14). Rat pups underwent neurobehavioural testing from birth to PD21 and were then sacrificed for neuropathologic assessment on PD21. Results: BUAL ligation resulted in growth restriction (IUGR) of the fetuses, which persisted throughout the study (p < 0.001). Reflex testing indicated IUGR pups were developmentally delayed compared to controls (p < 0.001). Open field testing on PD21 indicated hyperactivity in IUGR animals compared to controls (p < 0.001). Histological assessment showed a reduction in pyramidal cells in CA1 and CA3 of IUGR hippocampi and in myelin basic protein (MBP) immunohistochemistry signal. Broccoli sprout supplementation improved some reflex and behavioural measures, increased cell counts in CA1 and CA3 as well as MBP signal in growth restricted animals. Conclusions: Supplementation with broccoli sprouts during the last trimester of gestation and the first 2 weeks of life in the rat lessened the effects of chronic intra-uterine ischemia. These findings suggest a novel approach to the prevention of DD associated with perinatal HI.
Language
English
DOI
doi:10.7939/R32G8B
Rights
License granted by Amy Black (amy.black@ualberta.ca) on 2010-01-07T23:07:32Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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