ERA

Download the full-sized PDF of Characterization of hypertrophic scar formation in nude and knockout mice deficient in T, B and natural killer cellsDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3SW9C

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Characterization of hypertrophic scar formation in nude and knockout mice deficient in T, B and natural killer cells Open Access

Descriptions

Other title
Subject/Keyword
model
nude
hypertrophic
mouse
scar
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Momtazi, Moein
Supervisor and department
Tredget, Edward (Department of Surgery)
Anderson, Colin (Department of Surgery) (Department of Medicine and Medical Microbiology & Immunology)
Ding, Jie (Department of Surgery)
Examining committee member and department
Ding, Jie (Department of Surgery)
Tredget, Edward (Department of Surgery)
Elliott, John (Department of Medicine and Medical Microbiology & Immunology)
Anderson, Colin (Department of Surgery) (Department of Medicine and Medical Microbiology & Immunology)
Department
Department of Surgery
Specialization

Date accepted
2011-11-16T18:33:34Z
Graduation date
2012-06
Degree
Master of Science
Degree level
Master's
Abstract
Introduction: Hypertrophic scar (HSc) is a fibroproliferative disorder lacking a relevant animal model. Our objective is to characterize proliferative scars in human xenografts generated after grafting human skin onto mice deficient in T, B and natural killer cells. Methods: Nude, T-cell receptor (TCR) beta-/- delta-/-, RAG(recombination activating gene)-1-/- and RAG-2-/-gamma c-/- mice were xenografted with split thickness human skin. Controls were autografted with mouse skin. Animals were euthanized at 30, 60, 120 and 180 days postoperatively. Scar biopsies were subjected to hematoxylin and eosin, Masson’s trichrome, toluidine blue and picrosirius red staining. Immunohistochemistry included anti-human HLA-ABC, alpha–smooth muscle actin, decorin and biglycan staining. Results: Xenografting nude mice with human skin results in scars morphologically, histologically and immunohistochemically similar to human HSc. Knockout animals developed similar scars and demonstrate greater capacity for scar remodeling. Conclusion: Proliferative xenograft scars in knockout animals better represent the natural history of HSc. The role of activated, profibrotic fibrocytes and macrophages in these animals requires further investigation.
Language
English
DOI
doi:10.7939/R3SW9C
Rights
License granted by Moein Momtazi (momtazi@ualberta.ca) on 2011-11-10T22:28:34Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-04-24T23:01:43.412+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 202143764
Last modified: 2015:10:12 13:08:56-06:00
Filename: Momtazi_Moein_Spring 2012.pdf
Original checksum: ccf5bac92333ded79122c95ada5b19db
Well formed: true
Valid: true
File title: Microsoft Word - Master's Thesis.doc
File author: Moein Momtazi
Page count: 170
Activity of users you follow
User Activity Date