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Permanent link (DOI): https://doi.org/10.7939/R3P55DR10

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Arsenic Speciation Analysis in Environmental and Biological Systems Open Access

Descriptions

Other title
Subject/Keyword
arsenic speciation
high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICPMS)
Arsenicin A
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Chen, Lydia WL
Supervisor and department
Le, X. Chris
Examining committee member and department
Weinfeld, Michael (Oncology)
Harynuk, James (Chemistry)
Wang, Feiyue (Chemistry)
Loppnow, Glen (Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2013-01-03T11:32:02Z
Graduation date
2013-06
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
My thesis research focused on the development and application of analytical methods that enabled arsenic speciation in biological and environmental samples. A set of complementary chromatographic separation techniques were combined with inductively coupled plasma mass spectrometry and hydride generation. These techniques allowed for the separation and detection of arsenobetaine, arsenite, arsenate, monomethylarsonic acid, and dimethylarsinic acid. The application of these techniques to the determination of arsenic species in human urine has contributed to arsenic exposure measurement in a collaborative pilot epidemiological study. The application of a high performance liquid chromatography – inductively coupled plasma mass spectrometry technique showed that most of the groundwater samples from the Battersea Drain watershed located in southern Alberta had arsenic concentrations below the Canadian drinking water guideline value of 10 µg L-1. A set of complementary chromatographic separation techniques coupled with inductively coupled plasma mass spectrometry was developed to characterize a new arsenic species, Arsenicin A, previously reported for the presence in a marine sponge. These techniques enabled the separation and detection of an Arsenicin A model compound, arsenite, arsenate, monomethylarsonic acid, dimethylarsinic acid, arsenobetaine, and an arsenosugar. The application of these techniques to the determination of arsenic species in marine sponges suggested that arsenic speciation profile may be organism and habitat dependent. A comparative cellular uptake study that used human lung carcinoma A549 cells showed that these cells were able to uptake two orders of magnitude more Arsenicin A model compound than arsenite. The higher cellular uptake of Arsenicin A model compound was consistent with the higher toxicity of Arsenicin A model compound as compared to arsenite, suggesting that the cellular uptake is an important factor contributing to the toxicity of these arsenic species. My Ph.D. research has provided analytical techniques that are useful to environmental and biological studies.
Language
English
DOI
doi:10.7939/R3P55DR10
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Lydia W.L. Chen, Xiufen Lu, X. Chris Le. Analytica Chimica Acta 675(2010) 71-75

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