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Thin Films for Solid Matrix Laser Desorption/Ionization for Biomarker Analysis Open Access


Other title
Digital Microfluidics
Glancing Angle Deposition
Laser Desorption/Ionization
Thin Films
Surface Assisted Laser Desorption/Ionization
Alzheimer's Disease
Solid Matrix Laser Desorption/Ionization
Desorption Ionization on Silicon
Mass Spectrometry
Type of item
Degree grantor
University of Alberta
Author or creator
Peng, Chen
Supervisor and department
D. Jed Harrison (Chemistry)
Examining committee member and department
D. Jed Harrison (Chemistry)
Robert E. Campbell (Chemistry)
Thomas Thundat (Chemical and Materials Engineering)
Richard Oleschuk (Chemistry)
Julianne M. Gibbs-Davis (Chemistry)
Mark T. McDermott (Chemistry)
Department of Chemistry

Date accepted
Graduation date
Doctor of Philosophy
Degree level
Scientists and clinicians involved in the development of biomarkers confront with the challenge of selecting the best analytical methods among dozens that have been reported in the literature. Creation of new and improvement of exist technologies is an integral part of the pathway to analytical systems. The work of this thesis investigates new tools for biomarker analysis, which may also suggest biomarker discovery, using solid matrix assisted laser desorption/ionization mass spectrometry (SMALDI-MS) by using porous nanostructured films fabricated by the thin film process glancing angle deposition (GLAD). This project consists of three phases. The first objective of this thesis is to introduce a simple interface of DMF and GLAD, which provided an attractive proof-of-concept that GLAD is available for off-line SMALDI-MS detection. The second objective is to demonstrate a ready-to-use on-chip digestion system, which is coupled to SMALDI-MS for peptide fingerprinting mapping and is preliminary evaluated by digestion performance using a standard protein. The third objective is to investigate the performance of detecting free amino acids by modified silicon GLAD films based SMALDI. The application of GLAD nanostructured thin films to biomarker analysis SMALDI-MS is demonstrated and validated in this thesis.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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