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Permanent link (DOI): https://doi.org/10.7939/R3GT6R

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Dengue NS1 Detection using Chemically Modified Silicon Micropillars Open Access

Descriptions

Other title
Subject/Keyword
silicon micropillars
Dengue
covalent immobilization
micropillars chip based immunoassay
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Singh,Minashree
Supervisor and department
Kaur, Kamaljit (Faculty of Pharmacy and Pharmaceutical Sciences)
Mitra, Sushanta (Mechanical Engineering)
Examining committee member and department
Lavasanifar, Afsaneh (Faculty of Pharmacy and Pharmaceutical Sciences)
Kaur, Kamaljit (Faculty of Pharmacy and Pharmaceutical Sciences)
Mitra, Sushanta (Mechanical Engineering)
Department
Faculty of Pharmacy and Pharmaceutical Sciences
Specialization
Pharmaceutical Sciences
Date accepted
2012-09-27T13:55:40Z
Graduation date
2012-09
Degree
Master of Science
Degree level
Master's
Abstract
Dengue fever is a mosquito born viral disease that is reaching epidemic proportions. Despite of various control means, it is still flourishing and is becoming a major health concern around the world. In this thesis, a novel diagnosis method for the detection of Dengue virus has been developed. Micro-spot-integrated pillars chip (MSIP) has been used to increase the surface area for the immunoreaction and hence the probability of the Dengue virus detection. The MSIP chip used for the diagnosis of Dengue NS1 was chemically modified to immobilize the capture antibodies. The surface modification and the biofunctionalization of the capture antibody were done to increase the robustness and surface availability of the capture antibodies for the antibody-antigen reaction. Each step of the surface modification and biofunctionalization process was characterized by using different tools. The result showed that the method developed was five fold more sensitive than the conventional techniques.
Language
English
DOI
doi:10.7939/R3GT6R
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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