Non digestible carbohydrates in the diet determine toxicity of irinotecan (CPT-11)/5-fluorouracil in rats independently of β-glucuronidase activity in intestinal lumen

• Author / Creator

  Farhangfar, Arazm

• Aim of this study was to assess effects of non-digestible carbohydrates on the pathobiology of mucosal injury after chemotherapy.
  Rats bearing colon tumor (n=6/diet) were fed diets including 8% (w/w) as either cellulose, inulin, fructo-oligosaccharide (OligoF), 50:50 (w/w) inulin and oligoF (Synergy®), isomalto-oligosaccharide (IMO), type IV resistant starch (RS4) or cornstarch. Animals were treated with 2 cycles of CPT-11/5-fluorouracil.
  Animals fed RS4 or Synergy® were least ill with the least weight loss and anorexia, highest levels of gut associated lymphoid tissue (GALT) CD3+ and CD3CD8+ cells, least acute phase responses (haptoglobin, α-1-acid glycoprotein), least GALT OX6+ cells, CD152+ and CD71+ expression on CD8+ cells. Animals fed IMO or cornstarch were the most ill by these same criteria (p<0.05); animals fed inulin, cellulose and OligoF showed intermediate sickness.
  Dietary NDCHO determine chemotherapy toxicity in a controlled model of colon cancer treatment, this may be due to change in immune function.

• Subjects / Keywords

  • Irinotecan
  • Toxicity
  • Side effect
  • Adverse effect
  • Dietary fiber
  • NDCHO
  • Chemotherapy
  • CPT-11
  • Non-digestible carbohydrate

• Graduation date

  Fall 2012

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R3W13D

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.