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Heat tolerance mechanisms of an exceptional strain of Escherichia coli Open Access


Other title
Solute transport
Heat resistance
Escherichia coli
Stress response
Osmotic stress
Food safety
Type of item
Degree grantor
University of Alberta
Author or creator
Pleitner, Aaron M.
Supervisor and department
Gaenzle, Michael (Agricultural, Food and Nutritional Sciences)
McMullen, Lynn (Agricultural, Food and Nutritional Sciences)
Examining committee member and department
Chui, Linda (Laboratory Medicine and Pathology)
Department of Chemical and Materials Engineering

Date accepted
Graduation date
Master of Science
Degree level
The mechanism of heat resistance in an exceptionally resistant strain of E. coli was investigated. E. coli AW1.7 was compared to a heat sensitive strain, E. coli GGG10. The heat resistance of both strains was increased by inclusion of NaCl, but only E. coli AW1.7 exhibited a consistent heat increase to resistance when growth in NaCl concentrations ranging from 2 to 6%. The quantification of cytoplasmic solutes in E. coli and the determination of thermal ribosome and protein denaturation demonstrated that ribosomes are more stable in E. coli AW1.7 than in E. coli GGG10. Ribosome stability and heat resistance corresponded to the accumulation of compatible solutes. Differences in protein denaturation between the strains were not observed. In conclusion, heat resistance in E. coli AW1.7 is dependent on solute transport. Knowledge on the mechanism of heat resistance of E. coli will facilitate the design of novel intervention methods to warrant food safety.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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