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Use of surfaces functionalized with phage tailspike proteins to capture and detect bacteria in biosensors and bioassays Open Access


Other title
tailspike protein, bacteriophage, phage, P22, protein immobilization, biosensors, bioassays, bacterial detection, bacteria, salmonella
Type of item
Degree grantor
University of Alberta
Author or creator
Dutt, Sarang
Supervisor and department
Evoy, Stephane (Electrical and Computer Engineering)
Examining committee member and department
Szymanski, Christine (Biological Sciences)
Chen, Jie (Electrical and Computer Engineering)
Department of Electrical and Computer Engineering

Date accepted
Graduation date
Master of Science
Degree level
The food safety and human diagnostics markets are in need of faster working, reliable, sensitive, specific, low cost bioassays and biosensors for bacterial detection. This thesis reports the use of P22 bacteriophage tailspike proteins (TSP) immobilized on silanized silicon surfaces, roughened at a nano-scale, for specific capture and detection of Salmonella. Towards developing TSP biosensors, TSP immobilization characteristics were studied, and methods to improve bacterial capture were explored. Atomic force microscopy was used to count TSP immobilized on gold thin-films. Surface density counts are dependent on the immobilization scheme used. TSP immobilized on flat silicon (Si), silanized with 3-aminopropyltriethoxysilane and activated with glutaraldehyde, showed half the bacterial capture of gold thin-films. To improve bacterial capture, roughened mountain-shaped ridge-covered silicon (MSRCS) surfaces were coated with TSP and tested. Measurements of their bacterial surface density show that such MSRCS surfaces can produce bacterial capture close to or better than TSP-coated gold thin-films.
License granted by Sarang Dutt ( on 2010-06-17 (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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