ERA

Download the full-sized PDF of Immortalized human hepatocyte, an alternate model for the study of the propagation of HCV in vivo and in vitroDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3JQ6B

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Immortalized human hepatocyte, an alternate model for the study of the propagation of HCV in vivo and in vitro Open Access

Descriptions

Other title
Subject/Keyword
Immortalized human hepatocyte, HCV, SCID-uPA mice, de-immortalization, tamoxifen, Cre recombinase, JFH1, viral entry, HCV life cycle
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Mohajerani, Seyed Amir
Supervisor and department
Mason,Andrew(Medicine)
Douglas,Donna(Surgery)
Kneteman,Norman M( Surgery)
Examining committee member and department
Kneteman,Norman M( Surgery)
Mason,Andrew(Medicine)
Douglas,Donna(Surgery)
Department
Department of Surgery
Specialization

Date accepted
2010-12-02T18:38:19Z
Graduation date
2011-06
Degree
Master of Science
Degree level
Master's
Abstract
The chimeric Alb-uPA SCID mouse that has been transplanted with human hepatocytes is a model to facilitate in vivo study of HCV. We explored further development of the model by using repopulation with immortalized human hepatocytes (IHH) in place of primary human hepatocyte (PHH) transplantation to support HCV infection. In vitro HCV studies typically utilize a human hepatoma cell line (Huh7) and rely on transfection with transcribed genomic RNA derived from a unique HCV strain (JFH1). Unfortunately, this system has not been successful in support of infection with serum-derived HCV (HCVser). IHH may offer an alternative since their differentiation status remains close to that of PHH. IHH transfected with HCV RNA (H77 or JFH1) or infected with HCVser showed stable intracellular and supernatant HCV RNA by real-time RT-PCR. IHH showed intracellular HCV NS3 proteins. HCV transfected or infected IHH secrete infectious HCVcc for in vivo and vitro.
Language
English
DOI
doi:10.7939/R3JQ6B
Rights
License granted by Seyed Amir Mohajerani (sm44@ualberta.ca) on 2010-11-27T17:40:51Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-04-28T20:38:31.438+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1654317
Last modified: 2015:10:12 14:39:06-06:00
Filename: Mohajerani_Seyed Amir_Spring2011.pdf
Original checksum: c588c8d86732311a8f16aa3cf76ea6d1
Well formed: true
Valid: true
File title: Repopulation of SCID-uPA mouse model for HCV infection with conditionally immortalized human hepatocytes
File author: jlakey
Page count: 123
File language: en-US
Activity of users you follow
User Activity Date