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Permanent link (DOI): https://doi.org/10.7939/R3BV7B41N

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Theses and Dissertations

Fabrication of colloidal crystals featuring sub-20 nm pore size for protein separation Open Access

Descriptions

Other title
Subject/Keyword
Microfluidics
Protein separation
Electrophoresis
Crystal growth
Colloidal self-assembly
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Malekpourkoupaei, Ali
Supervisor and department
Harrison, D. Jed
Kostiuk, Larry W.
Examining committee member and department
Olfert, Jason (Mechanical Engineering)
Kostiuk, Larry W. (Mechanical Engineering)
Harrison, D. Jed (Chemistry)
Department
Department of Mechanical Engineering
Specialization

Date accepted
2012-09-24T14:19:32Z
Graduation date
2012-09
Degree
Master of Science
Degree level
Master's
Abstract
Clinical point-of-care (POC) diagnosis remains an open and challenging field of research worldwide in which the study of the mechanism and dynamics of protein molecule separation using electrophoresis is of considerable interest. The quality of a protein separation is strongly dependent on the separating matrix and conditions under which the electrophoresis is performed. This research is mainly focused on development of a novel separation matrix, based upon the selfassembly of particles in a colloidal suspension, within a microfluidic chip. Several challenges were overcome in developing crystalline particle arrays that generate a porous material with pores smaller than 20 nm. The effect of pore size on separation of proteins of differing molecular weight and size was then studied, and the mechanism of separation, which appears to change in the smallest size range, was explored.
Language
English
DOI
doi:10.7939/R3BV7B41N
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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