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Permanent link (DOI): https://doi.org/10.7939/R3Q814S0G

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Pbx homeodomain proteins pattern both the zebrafish retina and tectum Open Access

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Author or creator
French, C.R.
Erickson, T.
Callander, D.
Berry, K.M.
Koss, R.
Hagey, D.W.
Stout, J.
Wuennenberg-Stapleton, K.
Ngai, J.
Moens, C.B.
Waskiewicz, A.J.
Additional contributors
Subject/Keyword
embryonic-development
eye development
defects
homeobox genes
expression
regionalization
chick retina
hindbrain
morphogenesis
complexes
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Background Pbx genes encode TALE class homeodomain transcription factors that pattern the developing neural tube, pancreas, and blood. Within the hindbrain, Pbx cooperates with Hox proteins to regulate rhombomere segment identity. Pbx cooperates with Eng to regulate midbrain-hindbrain boundary maintenance, and with MyoD to control fast muscle cell differentiation. Although previous results have demonstrated that Pbx is required for proper eye size, functions in regulating retinal cell identity and patterning have not yet been examined. Results Analysis of retinal ganglion cell axon pathfinding and outgrowth in pbx2/4 null embryos demonstrated a key role for pbx genes in regulating neural cell behavior. To identify Pbx-dependent genes involved in regulating retino-tectal pathfinding, we conducted a microarray screen for Pbx-dependent transcripts in zebrafish, and detected genes that are specifically expressed in the eye and tectum. A subset of Pbx-dependent retinal transcripts delineate specific domains in the dorso-temporal lobe of the developing retina. Furthermore, we determined that some Pbx-dependent transcripts also require Meis1 and Gdf6a function. Since gdf6a expression is also dependent on Pbx, we propose a model in which Pbx proteins regulate expression of the growth factor gdf6a, which in turn regulates patterning of the dorso-temporal lobe of the retina. This, in concert with aberrant tectal patterning in pbx2/4 null embryos, may lead to the observed defects in RGC outgrowth. Conclusion These data define a novel role for Pbx in patterning the vertebrate retina and tectum in a manner required for proper retinal ganglion cell axon outgrowth.
Date created
2007
DOI
doi:10.7939/R3Q814S0G
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Rights
© 2007 French et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Citation for previous publication
French, C. R., Erickson, T., Callander, D., Berry, K. M., Koss, R., Hagey, D. W., Stout, J., et al. (2007). Pbx homeodomain proteins pattern both the zebrafish retina and tectum. BMC Developmental Biology, 7(1), 85. BioMed Central.
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