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Permanent link (DOI): https://doi.org/10.7939/R3S32S

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Theses and Dissertations

Regulation of vertebrate ladybird genes Open Access

Descriptions

Other title
Subject/Keyword
zebrafish
dorsoventral patterning
transcription factor
Tol2
enhancer
spinal cord
Ladybird
transgenic
Lbx1
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Lukowski, Chris
Supervisor and department
Waskiewicz, Andrew (Biological Sciences)
Examining committee member and department
Underhill, Alan (Medical Genetics)
Allison, William (Biological Sciences)
Pilgrim, David (Biological Sciences)
Department
Department of Biological Sciences
Specialization

Date accepted
2010-02-02T17:14:42Z
Graduation date
2010-06
Degree
Master of Science
Degree level
Master's
Abstract
Development of the vertebrate central nervous system is a complex process that relies on the accurate spatiotemporal distribution of signaling centers during embryogenesis. These signals provide cells with positional information, which is integrated via transcription factors and gene regulatory elements to generate a specific downstream gene expression profile that confers specific cellular functions. It is of interest to determine how cells acquire their unique spatiotemporal gene expression patterns. The wide variety of expression profiles established along the dorsoventral axis of the neural tube provides a great system to address this question. Recent advances in zebrafish transgenic technology, along with the phenomenon of a fish-specific genome duplication event, have been exploited here to provide an efficient way of identifying and characterizing gene regulatory elements. An identified neuronal-specific enhancer near the ladybird locus has been incorporated into a transgenic zebrafish strain driving fluorescent reporter protein expression in a subset of dorsal interneurons
Language
English
DOI
doi:10.7939/R3S32S
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: University of Alberta
File author: Chris Lukowski
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