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Evaluation of the Protection Induced by a Monotherapy of Anti-LFA-1 Monoclonal Antibody and Co-transplantation of Neonatal Porcine Islets with Sertoli Cells Open Access


Other title
Sertoli Cells
Neonatal Porcine Islets
Type of item
Degree grantor
University of Alberta
Author or creator
Bayrack, Kevin R
Supervisor and department
Rayat, Gina (Surgery)
Rajotte, Raymond (Surgery)
Examining committee member and department
Bleackley, R Chris (Biochemistry)
Rajotte, Raymond (Surgery)
Rayat, Gina (Surgery)
Department of Surgery
Experimental Surgery
Date accepted
Graduation date
Master of Science
Degree level
Two major barriers to islet transplantation are the need for an unlimited source of donor tissue and a safer method of immunosuppression. These may be overcome by xenotransplantation of neonatal porcine islets (NPI) along with combined co-transplantation of neonatal porcine Sertoli cells (SC) and transient use of anti-LFA-1 monoclonal antibody (mAb). In this study we aimed to identify potential mechanisms responsible for prolonged NPI islet xenograft survival with our combination therapy. Our data demonstrates that the combination of anti-LFA-1 mAb therapy along with the co-transplantation of SC is indeed highly efficacious in preventing NPI xenograft rejection as 20/27 treated mice achieved and maintained long-term graft survival. Although it appears that T regulatory cells are not solely responsible for maintaining long-term xenograft protection, they are likely important in establishing a TH2 cell phenotype and sharing a role with secreted SC products, such as serpina3n, in prolonging NPI xenograft protection.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Ramji QA, Bayrack K, Arefanian H, Marcet-Palacios M, Bleackley RC, Rajotte RV, Rayat GR. Protection of porcine islet xenografts in mice using sertoli cells and monoclonal antibodies. Transplantation 92(12): 1309-1315 (2011).

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