ERA

Download the full-sized PDF of The Use of Doxycycline in Asphyxiated NewbornsDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3V980106

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

The Use of Doxycycline in Asphyxiated Newborns Open Access

Descriptions

Other title
Subject/Keyword
Swine
Reactive oxygen species
Hypoxia-reoxygenation
Doxycycline
Matrix Metalloproteinase
Neonatal Asphyxia
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
LaBossiere, Joseph R
Supervisor and department
Bigam, David (Surgery)
Cheung, Po-Yin (Pharmacology/Surgery/Pediatrics)
Examining committee member and department
Cheung, Po-Yin (Pharmacology/Surgery/Pediatrics)
Bigam, David (Surgery)
Schulz, Richard (Pharmacology)
Churchill, Tom (Surgery)
Department
Department of Surgery
Specialization
Experimental Surgery
Date accepted
2013-09-19T10:26:31Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Asphyxia is a significant cause of newborn morbidity and mortality. Multiorgan injury and dysfunction is a common finding, mediated in part through an increase in matrix metalloproteinase (MMP) activity. We hypothesized administration of doxycycline, a known MMP inhibitor, would improve systemic and regional hemodynamics as well as attenuate myocardial, renal and intestinal injury and dysfunction in a clinically translatable newborn swine model of hypoxia-reoxygenation. Newborn piglets were subjected to hypoxia-reoxygenation and received normal saline (control) or one of three doses of doxycycline five minutes into resuscitation. Doxycycline improved recovery of cardiac index, stroke volume index, systemic arterial pressure (SAP), systemic oxygen delivery and consumption with no effect on heart rate. Pulmonary artery pressure (PAP) and PAP/SAP were reduced, while renal artery flow index and oxygen delivery were improved. Markers of myocardial, renal and intestinal injury were attenuated with doxycycline and associated with a reduction in tissue oxidative stress and total MMP-2 activity. We conclude that in a clinically translatable newborn swine model of hypoxia-reoxygenation, early administration of doxycycline during resuscitation improves systemic and regional hemodynamics, and attenuates myocardial, renal and intestinal injury.
Language
English
DOI
doi:10.7939/R3V980106
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-04-28T20:37:26.821+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 2596376
Last modified: 2015:10:12 14:39:06-06:00
Filename: LaBossiere_Joseph_Fall 2013.pdf
Original checksum: 63bbfccb680cb2663d19f694cf84e2be
Well formed: false
Valid: false
Status message: Invalid page tree node offset=154812
Status message: Unexpected error in findFonts java.lang.ClassCastException: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject cannot be cast to edu.harvard.hul.ois.jhove.module.pdf.PdfDictionary offset=3281
Status message: Invalid Annotation list offset=2535428
Status message: Outlines contain recursive references.
File title: List of Figures
Activity of users you follow
User Activity Date