ERA

Download the full-sized PDF of Ganglioside Increases Metastatic Potential and Susceptibility of Prostate Cancer to Gene Therapy in vitroDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R33M2R

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Ganglioside Increases Metastatic Potential and Susceptibility of Prostate Cancer to Gene Therapy in vitro Open Access

Descriptions

Other title
Subject/Keyword
adenovirus
gene therapy
metastasis
prostate cancer
ganglioside
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Miklavcic, John
Supervisor and department
Clandinin, M Tom (Agricultural, Food and Nutritional Science)
Examining committee member and department
Clandinin, M Tom (Agricultural, Food and Nutritional Science)
Hitt, Mary M (Experimental Oncology)
Mazurak, Vera C (Agricultural, Food and Nutritional Science)
Department
Department of Agricultural, Food and Nutritional Science
Specialization

Date accepted
2009-10-02T18:34:33Z
Graduation date
2009-11
Degree
Master of Science
Degree level
Master's
Abstract
Prostate cancer (CaP) is the 2nd most common cancer in North American men. Tumour management strategies are appropriate for early stage disease, but advanced disease has a poor prognosis and requires prompt treatment. Therefore, research into delay of tumour progression and efficacious treatment of aggressive cancer are of interest. Ganglioside was assessed for its role in altering markers of metastatic potential and susceptibility of CaP to adenovirus-mediated gene therapy. Healthy (RWPE-1) and malignant (DU-145, PC-3) prostate cells were cultured with or without mixed ganglioside. Differences in growth, ganglioside and integrin densities, and adenoviral infectivity were assessed between treatment and control groups. Ganglioside decreased (p<0.01) growth of PC-3 cells relative to untreated control. Ganglioside decreased (p<0.01) GD1a and increased (p<0.04) integrin densities in malignant prostate cells, suggesting ganglioside may increase metastatic potential of CaP. Ganglioside significantly increased adenovirus entry in PC-3 cells, thereby improving susceptibility of CaP to adenovirus-mediated gene therapy.
Language
English
DOI
doi:10.7939/R33M2R
Rights
License granted by John Miklavcic (miklavci@ualberta.ca) on 2009-10-01T21:59:17Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-04-29T16:21:04.201+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 508528
Last modified: 2015:10:12 10:37:44-06:00
Filename: Miklavcic_John_Fall 2009.pdf
Original checksum: 1bf27f0ed15e1c61836b796726e639c7
Well formed: true
Valid: true
Page count: 122
Activity of users you follow
User Activity Date