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Permanent link (DOI): https://doi.org/10.7939/R3X89D

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Developing experimental methods for identifying the sites of action of intraspinal microstimulation Open Access

Descriptions

Other title
Subject/Keyword
intraspinal microstimulation
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Christian, Breanne
Supervisor and department
Mushahwar, Vivian (Cell Biology)
Examining committee member and department
Campenot, Bob (Cell Biology)
Todd, Kathryn (Psychiatry)
Krukoff, Teresa (Cell Biology)
Department
Department of Cell Biology
Specialization

Date accepted
2011-05-17T15:23:25Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
Intraspinal microstimulation (ISMS) is a novel electrical stimulation approach to restore standing and ambulation in people with spinal cord injury. The technique entails inserting an array of microwires into the lumbosacral enlargement of the spinal cord to activate neuronal networks that control locomotion. Additionally, ISMS can be utilized to investigate the organization of these networks. In the present study, experimental methodology was developed to map the distribution of ISMS-activated neurons using immunohistochemistry to label c-Fos, an activity-dependent marker. The influence on c-Fos expression of the following conditions was studied: decerebration, laminectomy, microwire implantation, and ISMS. Data revealed that microwire implantation and decerebration minimally influenced c-Fos, while a laminectomy substantially increased c-Fos expression. Furthermore, results indicated that it is vital to monitor stimulation and adjust stimulus amplitude throughout the duration of stimulation. Using these data, a protocol was established that would aid in mapping the ISMS activated neuronal networks.
Language
English
DOI
doi:10.7939/R3X89D
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: Microsoft Word - ***BChristianMScThesis2011.formatted.rtf
File author: Breanne Christian
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