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Permanent link (DOI): https://doi.org/10.7939/R3QJ78630

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Vitamin D, an Immunomodulator for Eosinophils in Asthma and Allergy Open Access

Descriptions

Other title
Subject/Keyword
Asthma
Eosinophil
Vitamin D
Inflammation
Allergy
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Ethier, Caroline D
Supervisor and department
Cameron, Lisa (Department of Medicine)
Davoine, Francis (Department of Medicine and Faculte Saint-Jean)
Examining committee member and department
Lacy, Paige (Department of Medicine)
Cameron, Lisa (Department of Medicine)
Davoine, Francis (Department of Medicine and Faculte Saint-Jean)
Mazurak, Vera (Department of Agricultural, Food & Nutritional Science)
Department
Department of Medicine
Specialization
Experimental Medicine
Date accepted
2014-08-13T13:31:32Z
Graduation date
2014-11
Degree
Master of Science
Degree level
Master's
Abstract
Epidemiological studies correlate vitamin D deficiency with asthma severity. Calcitriol modulates receptor and cytokine expression from various leukocytes in an anti-inflammatory manner. Despite eosinophil activity during mucosal inflammation, knowledge on the direct effect of calcitriol on this granulocyte is very limited. We propose that calcitriol exerts direct effects on eosinophil biology by modulating its programmed cell death pathways and other functions. Purified peripheral blood eosinophils from atopic donors were incubated with calcitriol (10nM) for up to 14 days with and without IL-5. We observed that calcitriol significantly reduced eosinophil necrosis and cytolytic release of EPX in media when co-incubated with IL-5. The addition of calcitriol also significantly decreased CRTh2 and CCR10 expression on eosinophils. Eosinophil cytotoxic mediator release into mucosal tissues, resulting from necrotic cytolysis, as well as eosinophil recruitment to lungs are major contributors to airway inflammation in allergic asthma. Hence, the reduction of mucosal inflammation achieved by decreasing eosinophil necrosis and recruitment unveil new anti-inflammatory mechanisms for calcitriol in the context of airway inflammation.
Language
English
DOI
doi:10.7939/R3QJ78630
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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