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Development of Microfluidic Chips and a Customised Flow Control System for use in a Label-Free Cytometer Open Access


Other title
flow control system
biomedical instrumentation
microfludic chip design
flow cytometry
pressure driven flow
light scattering
label-free detection
Type of item
Degree grantor
University of Alberta
Author or creator
Ha, Debbie Feng Shan
Supervisor and department
Tsui, Ying Y. (Electrical and Computer Engineering)
Examining committee member and department
DeCorby, Ray (Electrical and Computer Engineering)
Rozmus, Wojciech (Physics)
Department of Electrical and Computer Engineering
Microsystems and Nanodevices
Date accepted
Graduation date
Master of Science
Degree level
Conventional cytometry uses fluorescently labelled cells that are laborious and costly to prepare. Using a label-free technique, individual cells can be differentiated by light scattering patterns, a result of different physical attributes. This thesis describes the development of a microfluidic label-free cytometer. The key components include a microfluidic chip, a laser light scattering system for cell characterization, and a microfluidic flow control system. A PDMS microfluidic chip was developed and described in detail. A customised flow control system with sorting functionality was constructed to respond faster than commercial pumps. The linear relationship between applied pump pressure and average flow speed was confirmed experimentally, as well as a sorting flow response of 48.3ms or less. Results from laser light scatter experiments with different biological cells were presented, where scatter patterns for each cell type were visually distinguishable.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: Introduction
File title: Optimization of Microfluidic Chip Design and Flow Control Mechanism for use in a Label-Free Cytometer
File author: Debbie Ha
Page count: 159
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