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Permanent link (DOI): https://doi.org/10.7939/R3CM6R

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Towards a portable and inexpensive lab-on-a-chip device for point of care applications Open Access

Descriptions

Other title
Subject/Keyword
sample preparation
lab on a chip
melting curve analysis
real time PCR
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Olanrewaju, Ayokunle Oluwafemi
Supervisor and department
Backhouse, Chris (Electrical and Computer Engineering)
Examining committee member and department
Hume (Bleoo), Stacey (Medical Genetics)
McMullin, James (Electrical and Computer Engineering)
Department
Department of Electrical and Computer Engineering
Specialization

Date accepted
2011-06-13T21:29:37Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
Ongoing work in the laboratory of Professor Chris Backhouse is aimed at developing a portable and inexpensive lab on a chip instrument. A system capable of molecular biology protocols including sample preparation (SP), polymerase chain reaction (PCR), and melting curve analysis (MCA) would meet the requirements for point of care genetic analysis. The SP, PCR, and MCA modules were designed and tested on a standalone basis and then integrated for analysis of raw clinical samples. An automated XY stage was developed for magnetic bead-based DNA purification. In addition, a LED/CCD-based optical detection module was employed for real time PCR and MCA. Data analysis algorithms and protocols were implemented to remove noise and interpret data. This work culminated in proof of principle on-chip SP-PCR-MCA to detect ß2m DNA from human buccal cells in a modular and inexpensive system.
Language
English
DOI
doi:10.7939/R3CM6R
Rights
License granted by Ayokunle Olanrewaju (ayokunle@ualberta.ca) on 2011-06-09T16:33:14Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: Contents
File title: Towards a portable and inexpensive lab on a chip device for point of care applications
File author: Ayo Olanrewaju
Page count: 163
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