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Expansion and Osteogenic Differentiation of Human Umbilical Cord Perivascular Stem Cells by Low Intensity Pulsed Ultrasound for Dentofacial Tissue Engineering Open Access


Other title
Stem cells
Low intensity pulsed ultrasound (LIPUS)
Human umbilical cord perivascular stem cell (HUCPV-SC)
Type of item
Degree grantor
University of Alberta
Author or creator
Aldosary, Tagreed
Supervisor and department
Doschak, Michael (Pharmacy)
Uludag, Hasan (Chemical Engineering)
El-Bialy, Tarek (Orthodontics)
Examining committee member and department
Eggert, Michael (Periodontics)
El-Bialy, Tarek (Orthodontics)
Doschak, Michael (Pharmacy)
Uludag, Hasan (Chemical Engineering)
Medical Sciences-Dentistry

Date accepted
Graduation date
Master of Science
Degree level
The objective of these experiments is to explore the effect of LIPUS on the ultraexpansion and osteogenic differentiation of harvested passage-4 HUCPV-SCs. HUCPV-SCs were divided into two groups: a treatment group that received LIPUS for 10 minutes for 1, 7, and 14 days and a control group that received a sham treatment utilizing both basic and osteogenic media. The results in basic media and osteogenic media demonstrated nonsignificant differences in cell count, ALP, DNA content, and CD90. Statistically significant expression of OSP and PCNA was observed on day 14 in LIPUS treated group. Nucleostemin expression in the LIPUS-treated group was insignificant on days 1 and 7. However, a selective increase in osteogenic markers was obtained on day 7 for ALP and OCN and on day 14 for OPN. Future experiments are required to explore the effects of different application times and/or techniques of LIPUS on the behaviour of HUCPV-SCs.
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