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Permanent link (DOI): https://doi.org/10.7939/R3GD0B

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Expansion and Osteogenic Differentiation of Human Umbilical Cord Perivascular Stem Cells by Low Intensity Pulsed Ultrasound for Dentofacial Tissue Engineering Open Access

Descriptions

Other title
Subject/Keyword
Stem cells
Low intensity pulsed ultrasound (LIPUS)
Human umbilical cord perivascular stem cell (HUCPV-SC)
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Aldosary, Tagreed
Supervisor and department
Doschak, Michael (Pharmacy)
Uludag, Hasan (Chemical Engineering)
El-Bialy, Tarek (Orthodontics)
Examining committee member and department
Eggert, Michael (Periodontics)
El-Bialy, Tarek (Orthodontics)
Doschak, Michael (Pharmacy)
Uludag, Hasan (Chemical Engineering)
Department
Medical Sciences-Dentistry
Specialization

Date accepted
2009-10-02T18:16:14Z
Graduation date
2009-11
Degree
Master of Science
Degree level
Master's
Abstract
The objective of these experiments is to explore the effect of LIPUS on the ultraexpansion and osteogenic differentiation of harvested passage-4 HUCPV-SCs. HUCPV-SCs were divided into two groups: a treatment group that received LIPUS for 10 minutes for 1, 7, and 14 days and a control group that received a sham treatment utilizing both basic and osteogenic media. The results in basic media and osteogenic media demonstrated nonsignificant differences in cell count, ALP, DNA content, and CD90. Statistically significant expression of OSP and PCNA was observed on day 14 in LIPUS treated group. Nucleostemin expression in the LIPUS-treated group was insignificant on days 1 and 7. However, a selective increase in osteogenic markers was obtained on day 7 for ALP and OCN and on day 14 for OPN. Future experiments are required to explore the effects of different application times and/or techniques of LIPUS on the behaviour of HUCPV-SCs.
Language
English
DOI
doi:10.7939/R3GD0B
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File author: Tagreed Aldosary
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