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Permanent link (DOI): https://doi.org/10.7939/R3JS9HK1G

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Genetics of Seed Coat and Stem Development in Flax (Linum usitatissimum L.) Open Access

Descriptions

Other title
Subject/Keyword
flax GASA-like gene
flax seed mucilage
flax seed color
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
To, Lai T
Supervisor and department
Deyholos, Micheal (Biological Sciences)
Examining committee member and department
Good, Allen (Biological Sciences)
Rahman, Habibur (Agricultural, Food & Nutritional Science)
Department
Department of Biological Sciences
Specialization
Plant Biology
Date accepted
2013-06-03T10:35:40Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Abstract Flax (linseed, Linum usitatissimum L) is cultivated either for its seeds or its stem (bast fiber). With the goal of contributing to development of novel flax germplasm, a forward genetics screen of EMS mutagenized flax (var. CDC Bethune) was conducted to identify seeds with abnormal pigmentation or mucilage. Seeds of 16,764 M4 families were examined visually, with and without staining by Toluidine Blue. A total of 86 putative seed color mutants were identified, with the most common phenotype being yellow seeds. There were 1,975 identified putative mucilage mutants, with a range of phenotypes, including Blue Seed, No Mucilage Released, and Abnormal Mucilage Released. A subset of the seed colors and mucilage phenotypes were tested for heritability. The seed colors were generally heritable, but the mucilage phenotypes appeared to show variable expressivity that is probably highly dependent on the environment. Histological analysis revealed how seed coat anatomy changes affected seed color, but did not explain the mucilage phenotypes tested. The lines have been provided to flax breeders other collaborators for further analysis and potential use in breeding programs. GASA (Gibberellic Acid Stimulated in Arabidopsis) genes encode a family of small proteins of unknown biochemical function, whose expression has been correlated with various processes in plants including hormone response, defense, and development. GASA genes have also been reported in our lab to be expressed in developing flax stems. To better characterize the GASA-like gene family in flax, I identified 20 predicted GASA-like genes in the flax whole genome assembly. Using microarray and qRT-PCR analysis, seven of these genes showed differential transcript expression during stem development. I cloned and verified the sequences of these genes. Based on these studies, I was able to define groups of flax GASA-like genes whose expression was well-correlated with stem segments in either the elongation or cell wall deposition stage. These results provide further evidence that specific GASA genes are involved in different processes in flax stem and/or fiber development.
Language
English
DOI
doi:10.7939/R3JS9HK1G
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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