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Permanent link (DOI): https://doi.org/10.7939/R3HW8H

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New chromatin regulators contributing to the transcriptional control of HUG1 Open Access

Descriptions

Other title
Subject/Keyword
chromatin
CAF-1
replication
Rtt106
replication-coupled nucleosome assembly
Asf1
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Walker, Amelia C
Supervisor and department
Schultz, Michael (Biochemistry)
Examining committee member and department
Berry, Fred (Medical genetics)
Schang, Luis (Biochemistry)
Schultz, Michael (Biochemistry)
Underhill, Alan (Medical genetics)
Department
Department of Biochemistry
Specialization

Date accepted
2012-05-16T16:07:28Z
Graduation date
2012-11
Degree
Master of Science
Degree level
Master's
Abstract
Chromatin structure is important aspect of transcriptional regulation. Replication-coupled (RC) nucleosome assembly is the process of depositing newly synthesized H3-H4 onto nascent DNA behind the replication fork, mediated by the histone chaperones Asf1, CAF-1, and Rtt106. The experiments described in thesis test our hypothesis that the RC chaperones contribute to the regulation of HUG1 due to the important role they play in RC nucleosome assembly and therefore chromatin structure. Collectively, research in this thesis provides evidence that CAF-1 and Rtt106 contribute to the repression of HUG1 in a way that is unrelated to its normal regulation. Interestingly, this repression does not involve Asf1, even though Asf1 functions upstream of these chaperones during RC nucleosome assembly. These results suggest divergent functions of the RC chaperones that differently affect the regulation of HUG1. These divergent functions of the RC chaperones also have opposing roles in promoting survival during prolonged replication stress.
Language
English
DOI
doi:10.7939/R3HW8H
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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