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Permanent link (DOI): https://doi.org/10.7939/R3XK7R

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The contribution of two phosphorylated surface modifications on the pathogenesis of Campylobacter upsaliensis Open Access

Descriptions

Other title
Subject/Keyword
phosphoramidate
pathogenesis
Campylobacter upsaliensis
serum suceptibility
phosphocholine
adherence and invasion
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Crowley, Shauna M
Supervisor and department
Szymanski, Christine M (Biological Sciences)
Examining committee member and department
Foley, Edan (Medical Microbiology & Immunology)
Dennis, Jon (Biological Sciences)
Lowary, Todd (Chemistry)
Department
Department of Biological Sciences
Specialization
Microbiology and Biotechnology
Date accepted
2012-08-28T13:48:42Z
Graduation date
2012-11
Degree
Master of Science
Degree level
Master's
Abstract
Campylobacter upsaliensis is a human pathogen most commonly associated with self-limiting gastroenteritis. Despite extensive epidemiological data indicating C. upsaliensis as an emerging pathogen, few studies have examined possible mechanisms of its virulence. The surface of C. upsaliensis is comprised of capsular polysaccharides (CPS) and lipooligosaccharides (LOS), which are likely involved in bacterial interactions with its host and environment. In this study, we demonstrated that the LOS of C. upsaliensis RM3195 is modified with phosphocholine (PCho) and its CPS is decorated with phosphoramidate (MeOPN) residues. These phosphorylated moieties are involved in host cell invasion potentially mediated through the platelet activating factor receptor. Also, PCho and MeOPN conversely affect bacterial survival in human serum; PCho increases C. upsaliensis susceptibility, while MeOPN provides protection. These results suggest that phosphorylated surface modifications play key roles in C. upsaliensis host survival as well as pathogenesis.
Language
English
DOI
doi:10.7939/R3XK7R
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Sørensen M.C.H., van Alphen L.B., Fodor C., Crowley S.M., Christensen B.B., Szymanski C.M. and Brøndsted L. (2012) Phase variable expression of capsular polysaccharide modifications allows Campylobacter jejuni to avoid bacteriophage infection in chickens. Front. Cell. Inf. Microbio. 2:11. doi: 10.3389/fcimb.2012.00011.

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