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Permanent link (DOI): https://doi.org/10.7939/R3P84442R

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Validation of Reagent-free GC Lipid Derivatization Method Using an Enzymatic Microreactor Open Access

Descriptions

Other title
Subject/Keyword
GC
lipid derivatization
microreactor
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Zhang, Yiran
Supervisor and department
Curtis, Jonathan (Agricutural, Food and Nutritional Science)
Examining committee member and department
McMullen, Lynn (Agricutural, Food and Nutritional Science)
Temelli, Feral (Agricutural, Food and Nutritional Science)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Food Science and Technology
Date accepted
2014-07-07T09:03:02Z
Graduation date
2014-11
Degree
Master of Science
Degree level
Master's
Abstract
Derivatization of fatty acids to produce volatile methyl or ethyl esters (FAME or FAEE) prior to GC analysis is an indispensable procedure in lipid analysis. A lipase immobilized porous polymer monolith microreactor was developed and shown to achieve online and quantitative conversion of triglycerides to FAEE. When in use, a low flow of oil in ethanol is passed through the 15cm long microreactor. Fully conversion of oil into FAEE will be achieved during the passage of the solution through the enzymatic microreactor, so that the products can be collected for direct GC analysis. Here we describe an optimization and a validation of the first generation microreactor for reagent-free derivatization of vegetable oil samples with varied fatty acid distributions. Results will be presented in order to demonstrate (i) artifact-free quantitative FAEE formation giving equivalent overall accuracy compared to AOCS method Ce 1k-09 for FAME; (ii) microreactor intermediate precision and reusability; (iii) achieve conversion in a shorter time. These attributes are required for a future automated system.
Language
English
DOI
doi:10.7939/R3P84442R
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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