ERA

Download the full-sized PDF of Orally Administered P22 Phage Tailspike Protein Reduces Salmonella Colonization in Chickens: Prospects of a Novel Therapy against Bacterial InfectionsDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R32C3D

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Biological Sciences, Department of

Collections

This file is in the following collections:

Journal Articles (Biological Sciences)

Orally Administered P22 Phage Tailspike Protein Reduces Salmonella Colonization in Chickens: Prospects of a Novel Therapy against Bacterial Infections Open Access

Descriptions

Author or creator
Waseh, S.
Hanifi-Moghaddam, P.
Coleman, R.
Masotti, M.
Ryan, S.
Foss, M.
MacKenzie, R.
Henry, M.
Szymanski, C.M.
Tanha, J.
Additional contributors
Aziz, R.K.
Subject/Keyword
Escherichia-coli strains
base-plate parts
beta-helix domain
gastrointestinal-tract
Enterica serovar typhimurium
crystal-structure
monoclonal-antibodies
Clostridium-difficile
O-antigen
head-binding domain
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
One of the major causes of morbidity and mortality in man and economically important animals is bacterial infections of the gastrointestinal (GI) tract. The emergence of difficult-to-treat infections, primarily caused by antibiotic resistant bacteria, demands for alternatives to antibiotic therapy. Currently, one of the emerging therapeutic alternatives is the use of lytic bacteriophages. In an effort to exploit the target specificity and therapeutic potential of bacteriophages, we examined the utility of bacteriophage tailspike proteins (Tsps). Among the best-characterized Tsps is that from the Podoviridae P22 bacteriophage, which recognizes the lipopolysaccharides of Salmonella enterica serovar Typhimurium. In this study, we utilized a truncated, functionally equivalent version of the P22 tailspike protein, P22sTsp, as a prototype to demonstrate the therapeutic potential of Tsps in the GI tract of chickens. Bacterial agglutination assays showed that P22sTsp was capable of agglutinating S. Typhimurium at levels similar to antibodies and incubating the Tsp with chicken GI fluids showed no proteolytic activity against the Tsp. Testing P22sTsp against the three major GI proteases showed that P22sTsp was resistant to trypsin and partially to chymotrypsin, but sensitive to pepsin. However, in formulated form for oral administration, P22sTsp was resistant to all three proteases. When administered orally to chickens, P22sTsp significantly reduced Salmonella colonization in the gut and its further penetration into internal organs. In in vitro assays, P22sTsp effectively retarded Salmonella motility, a factor implicated in bacterial colonization and invasion, suggesting that the in vivo decolonization ability of P22sTsp may, at least in part, be due to its ability to interfere with motility… Our findings show promise in terms of opening novel Tsp-based oral therapeutic approaches against bacterial infections in production animals and potentially in humans.
Date created
2010
DOI
doi:10.7939/R32C3D
License information
© 2010 Waseh et al. This is an open-access...
Rights
© 2010 Waseh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation for previous publication
Waseh, S., Hanifi-Moghaddam, P., Coleman, R., Masotti, M., Ryan, S., Foss, M., MacKenzie, R., et al. (2010). Orally Administered P22 Phage Tailspike Protein Reduces Salmonella Colonization in Chickens: Prospects of a Novel Therapy against Bacterial Infections. (R. K. Aziz, Ed.)PLoS ONE, 5(11), 9.
Source
Link to related item

File Details

Date Uploaded
Date Modified
2014-04-25T00:22:46.319+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 831299
Last modified: 2015:10:12 12:34:01-06:00
Filename: PLoS_One_5_2010_e13904.pdf
Original checksum: d4d072960458693c29decf0f0b8758ad
Well formed: true
Valid: true
File title: pone.0013904 1..9
Page count: 9
Activity of users you follow
User Activity Date