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Inheritance of peroxisomes in the yeast Yarrowia lipolytica Open Access


Other title
Yarrowia lipolytica
Type of item
Degree grantor
University of Alberta
Author or creator
Chang, Jinlan
Supervisor and department
Rachubinski, Richard (Cell Biology)
Examining committee member and department
Wang, Zhixiang (Cell Biology)
Mullen, Robert (University of Guelph)
Casey, Joseph (Physiology)
Eitzen, Gary (Cell Biology)
Department of Cell Biology

Date accepted
Graduation date
Doctor of Philosophy
Degree level
Peroxisomes are indispensable organelles that perform many essential metabolic activities. Thus, eukaryotic cells have evolved molecular mechanisms to ensure the inheritance of peroxisomes from mother cell to daughter cell at cell division. In the budding yeast Saccharomyces cerevisiae, the class V myosin motor, Myo2p, interacts with its peroxisomal receptor, Inp2p, to move peroxisomes along actin from mother cell to bud, while the peroxisomal membrane protein Inp1p functions to tether peroxisomes to the cell cortex. In this thesis, I report the results of investigations of peroxisome inheritance using the dimorphic yeast Yarrowia lipolytica as a model system. We showed that peroxisome mobility and inheritance are dependent on actin in Y. lipolytica. Interrogation of the Y. lipolytica genome revealed one class V myosin. This myosin V is involved in transporting peroxisomes from mother cell to bud. We characterized YlInp1p, the othologue of S. cerevisiae Inp1p, as the first peroxisomal protein required for peroxisome inheritance in Y. lipolytica. We demonstrated that YlInp1p functions to anchor peroxisomes in both mother cell and bud. YlInp1p has an additional role in the dimorphic transition from the yeast form to the hyphal form in Y. lipolytica. We identified Pex3Bp, a paralogue of Pex3p, as the peroxisome-specific receptor for myosin V in Y. lipolytica. Pex3Bp interacts directly with the globular tail of myosin V. Pex3Bp also interacts with itself and with Pex3p. In cells lacking Pex3Bp, peroxisomes are preferentially retained in the mother cell, while the majority of peroxisomes gather and are transferred to the bud in cells overproducing Pex3Bp. Overexpression of PEX3 can partially complement the phenotype of pex3B∆ cells, while overexpression of PEX3B cannot complement the phenotype of pex3∆ cells. Interestingly, Pex3p, which has been shown previously to function in the de novo formation of peroxisomes from the ER, also interacts directly with the globular tail of myosin V. Therefore, Pex3p is involved in peroxisome inheritance. In addition, cells lacking Pex3Bp contain hyperelongated, tubulo-reticular peroxisomes, indicating that Pex3Bp has a role in peroxisome morphology. Our findings suggest that both Pex3Bp and Pex3p are multifunctional proteins that are involved in different steps of the peroxisome biogenic cascade.
License granted by Jinlan Chang ( on 2010-04-28T16:24:12Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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