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Permanent link (DOI): https://doi.org/10.7939/R3901ZS6T

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Expression and purification of recombinant forms of two Arabidopsis thaliana PR-10 homologues (MLP423 and Bet v 1) Open Access

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Other title
Subject/Keyword
Bet v 1
PR-10 homologues
MLP423
Arabidopsis
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
MUHAMMAD, SAJAD
Supervisor and department
Professor Nat Kav, Department of Agricultural, Food and Nutritional Science, University of Alberta
Examining committee member and department
Professor Randall Weselake, Department of Agricultural, Food and Nutritional Science, University of Alberta
Professor Stephen Strelkov, Department of Agricultural, Food and Nutritional Science, University of Alberta
Professor Nat Kav, Department of Agricultural, Food and Nutritional Science, University of Alberta
Professor Michael Deyholos, Department of Biological Sciences, University of Alberta
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Plant Science
Date accepted
2013-06-12T14:20:04Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Pathogenesis related 10 (PR-10) proteins have important roles in mediating plant abiotic and biotic stress tolerance. Most of the members of this family possess ribonuclease activity. The homologues of PR-10 (ABR17) protein, MLP423 and Bet v 1, may have similar biological and physiological functions. The deduced amino acid sequence of MLP423 and Bet v 1, when compared to ABR17, indicated several conserved amino acids and a conserved P-loop motif (GxGGxGxxK). In the present investigation, cDNAs encoding Arabidopsis thaliana MLP423 and Bet v 1 were isolated and cloned, recombinant proteins expressed in E. coli, and purified using Ni-NTA and size exclusion chromatography. Both in-solution and in-gel RNase activity assays were conducted to determine whether these recombinant proteins possessed RNase activity, which indicated that neither MLP423 nor Bet v 1 possessed RNase activity. Our results are discussed in the context of the possible functions of these PR-10 homologues in plants.
Language
English
DOI
doi:10.7939/R3901ZS6T
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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