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Gangliosides in Plasma and Chylomicrons of Control Participants and Individuals with Diabetes Fed both a High- and Low-Fat Diet Open Access


Other title
feeding trial
type 2 diabetes
Type of item
Degree grantor
University of Alberta
Author or creator
Beveridge, Michelle H
Supervisor and department
Clandinin, Tom (Nutrition and Metabolism)
McCargar, Linda (Human Nutrition)
Examining committee member and department
Ryan, Edmond (Endocrinology)
Mazurak, Vera (Nutrition and Metabolism)
McCargar, Linda (Human Nutrition)
Clandinin, Tom (Nutrition and Metabolism)
Department of Agricultural, Food, and Nutritional Science
Nutrition and Metabolism
Date accepted
Graduation date
Master of Science
Degree level
Aim: To investigate whether the plasma and chylomicron ganglioside profiles of participants with type 2 diabetes differ from those of matched control participants at 0, 2, 4, 6 and 8 hours postprandial when individuals are fed both a high- and low-fat diet for three days each. Background: Type 2 diabetes is a disease that impacts a growing portion of the population increasing the risk of cardiovascular disease, and leading to early mortality. Previous studies have reported that gangliosides, bioactive lipids, specifically GM3, can lead to the insulin receptor dissociating from the caveolae-1 complex in the cell membrane in adipocytes. Mice that have GM3 synthase knocked out have increased insulin sensitivity. Methods: In the present study, plasma samples from matched individuals with type 2 diabetes and control participants were fed both a high- and low-fat diet for three days each in a randomized cross-over controlled study. Plasma samples from 0, 2, 4, 6, and 8 hours postprandial, were collected, extracted and analyzed. Chylomicron samples from 0 and 4 hours were also analyzed. Gangliosides were extracted from plasma/chylomicron samples using a Folch technique and analyzed using a triple quad mass spectrometer. Result: The overall trend in both the plasma and chylomicron data indicated that there was no significant difference between ganglioside content in the plasma of individuals who were fed both a high- and low-fat diet in either the control participants or participants with diabetes. However, the majority of the plasma data showed a significant difference between ganglioside content over collection timepoints. Conclusion: The results indicate that there is no significant difference in ganglioside profiles of non-diabetic participants and participants with diabetes, matched for age, gender and body mass index, or that a high- vs low-fat diet influences plasma/chylomicron ganglioside profiles.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Wilke M. Dietary fat, plasma triglycerides and de novo lipogenesis in type 2 diabetes [Dissertation]. Edmonton (AB): University of Alberta; 2008.

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