ERA

Download the full-sized PDF of Designing a point-of-care detection assay for tuberculosisDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3236S

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Designing a point-of-care detection assay for tuberculosis Open Access

Descriptions

Other title
Subject/Keyword
Chemical treatment of serum sample
Tuberculosis
Bispecific monoclonal antibody
Point of care assay
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Sarkar, Susmita
Supervisor and department
Suresh, Mavanur R (Pharmaceutical Sciences)
Examining committee member and department
Suresh, Mavanur R (Pharmaceutical Sciences)
Loebenberg, Raimar (Pharmaceutical Sciences)
Lowary, Todd L (Chemistry)
Department
Faculty of Pharmacy and Pharmaceutical Sciences
Specialization

Date accepted
2011-07-08T15:03:43Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
The current status of the disease of tuberculosis (TB) demands a rapid, simple and inexpensive point-of-care detection. This study reported the designing of an enzyme linked immunoassay that could detect the antigen lipoarabinomannan (LAM), found to be present in different body fluids of the infected individuals. Initially, a bispecific monoclonal antibody was developed. It was subsequently used to design the sandwich immunoassay using the swabs. The limit of detection for spiked synthetic LAM was found to be 5.0 ng/ml (bovine urine), 0.5 ng/ml (rabbit serum) and 0.005 ng/ml (saline) and that for bacterial LAM from M. tuberculosis H37Rv was found to be 0.5 ng/ml (rabbit serum). Finally the assay was evaluated using the stored clinical samples collected from the TB patients. In addition to good sensitivity, the end point could be read visually within two hours of sample collection.
Language
English
DOI
doi:10.7939/R3236S
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-04-29T17:04:52.088+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1555738
Last modified: 2015:10:12 10:45:53-06:00
Filename: Sarkar_Susmita_Fall 2011.pdf
Original checksum: 44c99661701a44a0d3b0d1633b7d587b
Well formed: true
Valid: true
File title: Microsoft Word - Thesis _complete_.doc
File author: drbgrp
Page count: 146
Activity of users you follow
User Activity Date