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Permanent link (DOI): https://doi.org/10.7939/R3X94V

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Development of a sulfate microelectrode for profiling environmental biofilms Open Access

Descriptions

Other title
Subject/Keyword
Biofilm
Microelectrode
Sulfate
Microsensor
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Ren, Shujie
Supervisor and department
Yu, Tong (Civil & Environmental Engineering)
Examining committee member and department
Zeng, Hongbo (Chemical & Materials Engineering)
Buchanan, Ian (Civil & Environmental Engineering)
Department
Department of Civil and Environmental Engineering
Specialization

Date accepted
2011-09-21T18:52:05Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
Microelectrodes can be used for profiling environmental biofilms. Currently there is no sulfate microelectrode available for studying sulfate reduction in biofilms. In this study, a sulfate microelectrode was fabricated and tested in measurement of environmental biofilms for the first time. The composition of the sulfate-sensing membrane in the microelectrode was optimized. The sulfate microelectrode, with a tip diameter of 20 micrometres, displayed satisfying selectivity to SO42-. It exhibited log-linear response to sulfate concentration change with a Nernstian slope of -25.8 mV per concentration decade. The linear detection range was 10-5 M to 10-2 M SO42- with a detection limit of 10-5 M SO42- in water. The response time was 90 seconds. The sulfate microelectrode was applied on environmental biofilm samples. The sulfate profile obtained indicated the sulfate concentration change inside the biofilm. In conclusion, a sulfate microelectrode with satisfying characteristics was fabricated successfully and was useful for characterizing environmental biofilms.
Language
English
DOI
doi:10.7939/R3X94V
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: Development of a Sulfate Microelectrode for Profiling Environmental Biofilms
File author: S.Ren
Page count: 59
File language: zh-CN
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