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Permanent link (DOI): https://doi.org/10.7939/R30S15
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Pollen-mediated gene flow from transgenic safflower ( Carthamus tinctorius L.) intended for plant molecular farming to conventional safflower Open Access
- Author or creator
McPherson, Marc A.
Good, Allen G.
Topinka, A. Keith C.
McKenzie, Ross H.
Cathcart, R. Jason
Christianson, Jed A.
Hall, Linda M.
- Additional contributors
plant molecular farming
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- Journal Article (Published)
Field experiments were conducted in Chile and western Canada to measure short-distance (0 to 100 m) outcrossing from transgenic safflower (Carthamus tinctorius L.) intended for plant molecular farming to nontransgenic commodity safflower of the same variety. The transgenic safflower used as the pollen source was transformed with a construct for seed-specific expression of a high-value protein and constitutive expression of a gene conferring resistance to the broad-spectrum herbicide glufosinate. Progeny of non-transgenic plants grown in plots adjacent to the transgenic pollen source were screened for glufosinate resistance to measure outcrossing frequency. Outcrossing frequency differed among locations: values closest to the transgenic pollen source (0 to 3 m) ranged from 0.48 to 1.67% and rapidly declined to between 0.0024 to 0.03% at distances of 50 to 100 m. At each location, outcrossing frequency was spatially heterogeneous, indicating insects or wind moved pollen asymmetrically. A power analysis assuming a binomial distribution and a range of alpha values (type 1 error) was conducted to estimate an upper and lower confidence interval for the probable transgenic seed frequency in each sample. This facilitated interpretation when large numbers of seeds were screened from the outcrossing experiments and no transgenic seeds were found. This study should aid regulators and the plant molecular farming industry in developing confinement strategies to mitigate pollen mediated gene flow from transgenic to non-transgenic safflower.
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- Copyright ISBR, EDP Sciences, 2009
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Marc A. McPherson, Allen G. Good, A. Keith C. Topinka, Rong-Cai Yang, Ross H. McKenzie, R. Jason Cathcart, Jed A. Christianson, Curtis Strobeck and Linda M. Hall (2009). Pollen-mediated gene flow from transgenic safflower ( Carthamus tinctorius L.) intended for plant molecular farming to conventional safflower. Environmental Biosafety Research, 8 , pp 19-32 doi:10.1051/ebr/2008023
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File title: Introduction
File title: Pollen-mediated gene flow from transgenic safflower \(Carthamus tinctorius L.\) intended for plant molecular farming to conventional safflower
File author: McPherson Marc A., Good Allen G., Topinka A. Keith C., Yang Rong-Cai, McKenzie Ross H., Cathcart R. Jason, Christianson Jed A., Strobeck Curtis, Hall Linda M.
Page count: 14