ERA

Download the full-sized PDF of Pollen-mediated gene flow from transgenic safflower ( Carthamus tinctorius L.) intended for plant molecular farming to conventional safflowerDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R30S15

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Biological Sciences, Department of

Collections

This file is in the following collections:

Journal Articles (Biological Sciences)

Pollen-mediated gene flow from transgenic safflower ( Carthamus tinctorius L.) intended for plant molecular farming to conventional safflower Open Access

Descriptions

Author or creator
McPherson, Marc A.
Good, Allen G.
Topinka, A. Keith C.
Yang, Rong-Cai
McKenzie, Ross H.
Cathcart, R. Jason
Christianson, Jed A.
Strobeck, Curtis
Hall, Linda M.
Additional contributors
Subject/Keyword
vicinism
power analysis
safflower
gene-flow patterns
binomial distribution
plant molecular farming
outcrossing
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Field experiments were conducted in Chile and western Canada to measure short-distance (0 to 100 m) outcrossing from transgenic safflower (Carthamus tinctorius L.) intended for plant molecular farming to nontransgenic commodity safflower of the same variety. The transgenic safflower used as the pollen source was transformed with a construct for seed-specific expression of a high-value protein and constitutive expression of a gene conferring resistance to the broad-spectrum herbicide glufosinate. Progeny of non-transgenic plants grown in plots adjacent to the transgenic pollen source were screened for glufosinate resistance to measure outcrossing frequency. Outcrossing frequency differed among locations: values closest to the transgenic pollen source (0 to 3 m) ranged from 0.48 to 1.67% and rapidly declined to between 0.0024 to 0.03% at distances of 50 to 100 m. At each location, outcrossing frequency was spatially heterogeneous, indicating insects or wind moved pollen asymmetrically. A power analysis assuming a binomial distribution and a range of alpha values (type 1 error) was conducted to estimate an upper and lower confidence interval for the probable transgenic seed frequency in each sample. This facilitated interpretation when large numbers of seeds were screened from the outcrossing experiments and no transgenic seeds were found. This study should aid regulators and the plant molecular farming industry in developing confinement strategies to mitigate pollen mediated gene flow from transgenic to non-transgenic safflower.
Date created
2009
DOI
doi:10.7939/R30S15
License information
Rights
Copyright ISBR, EDP Sciences, 2009
Citation for previous publication
Marc A. McPherson, Allen G. Good, A. Keith C. Topinka, Rong-Cai Yang, Ross H. McKenzie, R. Jason Cathcart, Jed A. Christianson, Curtis Strobeck and Linda M. Hall (2009). Pollen-mediated gene flow from transgenic safflower ( Carthamus tinctorius L.) intended for plant molecular farming to conventional safflower. Environmental Biosafety Research, 8 , pp 19-32 doi:10.1051/ebr/2008023
Source
Link to related item

File Details

Date Uploaded
Date Modified
2014-04-28T22:52:07.737+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 270119
Last modified: 2015:10:12 10:28:27-06:00
Filename: EBR_8_2009_19.pdf
Original checksum: e24c5b95a4e206886eaa88c9da59f27b
Well formed: true
Valid: false
Status message: Invalid destination object offset=194004
File title: Introduction
File title: Pollen-mediated gene flow from transgenic safflower \(Carthamus tinctorius L.\) intended for plant molecular farming to conventional safflower
File author: McPherson Marc A., Good Allen G., Topinka A. Keith C., Yang Rong-Cai, McKenzie Ross H., Cathcart R. Jason, Christianson Jed A., Strobeck Curtis, Hall Linda M.
Page count: 14
Activity of users you follow
User Activity Date