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Modeling the transport of cryoprotective agents in articular cartilage for cryopreservation Open Access

Descriptions

Other title
Subject/Keyword
Transport
Cryopreservation
Biomechanical model
Articular cartilage
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Abazari Torqabeh, Alireza
Supervisor and department
Elliott, Janet AW (Chemical and Materials Engineering)
McGann, Locksley E (Laboratory Medicine and Pathology)
Examining committee member and department
Finlay, Warren (Mechanical Engineering)
Masliyah, Jacob (Chemical and Materials Engineering)
Pegg, David (Biology Department, York University, York, UK)
Thompson, Richard (Biomedical Engineering)
Department
Department of Chemical and Materials Engineering
Specialization

Date accepted
2010-11-29T21:53:02Z
Graduation date
2011-06
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Loading vitrifiable concentrations of cryoprotective agents is an important step for cryopreservation of biological tissues by vitrification for research and transplantation purposes. This may be done by immersing the tissue in a cryoprotective agent (CPA) solution, and increasing the concentration, continuously or in multiple steps, and simultaneously decreasing the temperature to decrease the toxicity effects of the cryoprotective agent on the tissue cellular system. During cryoprotective agent loading, osmotic water movement from the tissue to the surrounding solution, and the resultant tissue shrinkage and stress-strain in the tissue matrix as well as on the cellular system can significantly alter the outcome of the cryopreservation protocol. In this thesis, a biomechanical model for articular cartilage is developed to account for the transport of the cryoprotective agent, the nonideal-nondilute properties of the vitrifiable solutions, the osmotic water movement and the resultant tissue shrinkage and stress-strain in the tissue matrix, and the osmotic volume change of the chondrocytes, during cryoprotective agent loading in the cartilage matrix. Four essential transport parameters needed for the model were specified, the values of which were obtained uniquely by fitting the model to experimental data from porcine articular cartilage. Then, it was shown that using real nonuniform initial distributions of water and fixed charges in cartilage, measured separately in this thesis using MRI, in the model can significantly affect the model predictions. The model predictions for dimethyl sulfoxide diffusion in porcine articular cartilage were verified by comparing to spatially and temporally resolved measurements of dimethyl sulfoxide concentration in porcine articular cartilage using a spectral MRI technique, developed for this purpose and novel to the field of cryobiology. It was demonstrated in this thesis that the developed mathematical model provides a novel tool for studying transport phenomena in cartilage during cryopreservation protocols, and can make accurate predictions for the quantities of interest for applications in the cryopreservation of articular cartilage.
Language
English
DOI
doi:10.7939/R37Q7X
Rights
License granted by Alireza Abazari Torqabeh (aa1@ualberta.ca) on 2010-11-26T22:30:15Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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